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This volume is a valuable and timely resource for a broad audience with interests in basic and translational cancer biology, cancer drug development, as well as in the practice of personalized oncology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cancer Gene Networks aims to ensure successful results in the further study of this evolving and vital field. Ultimately these efforts will guide development of transformative strategies for cancer diagnosis and treatment.
Introduction- Cancer Gene Networks.- Emerging Methods in Chemoproteomics with Relevance to Drug Discovery.- ANXA7-GTPase as Tumor Suppressor: Mechanisms and Therapeutic Opportunities.- Experimental and Study Design Considerations for Uncovering Oncometabolites.- Targeting Deubiquitinating Enzymes and Autophagy in Cancer.- Quantitative Clinical Imaging Methods for Monitoring Intratumoral Evolution.- Transcriptome and Proteome Analyses of TNFAIP8 Knockdown Cancer Cells Reveal New Insights into Molecular Determinants of Cell Survival and Tumor Progression.- Network-oriented Approaches to Anti-cancer Drug Response.- CRISPR/Cas-mediated Knockin in Human Pluripotent Stem Cells.- Complete Transcriptome RNA-Seq.- Computational Methods and Correlation of Exon-skipping Events with Splicing, Transcription and Epigenetic Factors.- Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma.- microRNA Target Prediction.- Evaluating the Delivery of Proteins to the Cytosol of Mammalian Cells.- Validation of Biomarker Proteins using Reverse Capture Protein Microarrays.- Chemical Synthesis of Activity-Based Diubiquitin Probes.- Profiling the Dual Enzymatic Activities of the Serine/Threonine Kinase IRE1a.
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