Plant Cold Acclimation

TRAMA

This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.

SOMMARIO

1. Introduction: Plant Cold Acclimation and Winter Survival             Dirk K. Hincha and Ellen Zuther   2. Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays             Anja Thalhammer, Majken Pagter, Dirk K. Hincha, and Ellen Zuther   3. Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements             Andrey V. Malyshev, Ilka Beil, and Juergen Kreyling   4. Infrared Thermal Analysis of Plant Freezing Processes             Gilbert Neuner and Edith Kuprian   5. Conducting Field Trials for Frost Tolerance Breeding in Cereals             Luigi Cattivelli and Cristina Crosatti   6. A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes             Annick Bertrand, Annie Claessens, Josée Bourassa, Solen Rocher, and Vern Baron   7. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation             Evelyne Téoulé and Carine Géry   8. Identification of Arabidopsis Mutants with Altered Freezing Tolerance             Carlos Perea-Resa, Rafael Catalá, and Julio Salinas   9. Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues             Seizo Fujikawa and Keita Endoh   10. Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue             David P. Livingston III and Tan D. Tuong   11. Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography             Ian R. Willick, Jarvis Stobbs, Chithra Karunakaran, and Karen K. Tanino   12. Proteomic Approaches to Identify Proteins Responsive to Cold Stress             Anna M. Jozefowicz, Stefanie Döll, and Hans-Peter Mock   13. Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins             Kamal Md. Mostafa, Daisuke Takahashi, Takato Nakayama, Yushi Miki, Yukio Kawamura, and Matsuo Uemura   14. A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition             Yu Song, Hieu Sy Vu, Sunitha Shiva, Carl Fruehan, Mary R. Roth, Pamela Tamura, and Ruth Welti   15. Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry             Alexander Erban, Federico Martinez-Seidel, Yogeswari Rajarathinam, Frederik Dethloff, Isabel Orf, Ines Fehrle, Jessica Alpers, Olga Beine-Golovchuk, and Joachim Kopka   16. Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation             Andras Bittner, Thomas Griebel, Jörn van Buer, Ilona Juszczak-Debosz, and Margarete Baier   17. Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation             Daisuke Takahashi, Ellen Zuther, and Dirk K. Hincha   18. Subcellular Compartmentation of Metabolites Involved in Cold Acclimation             Imke I. Hoermiller, Thomas Nägele, and Arnd G. Heyer   19. Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime             Lisa Fürtauer and Thomas Nägele   20. Characterization of Ice Binding Proteins from Sea-Ice Microalgae             Maddalena Bayer-Giraldi, EonSeon Jin, and Peter W. Wilson   21. Isolation and Characterization of Ice-Binding Proteins from Higher Plants             Melissa Bredow, Heather E. Tomalty, Laurie A. Graham, Audrey K. Gruneberg, Adam J. Middleton, Barbara Vanderbeld, Peter L. Davies, and Virginia K. Walker

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071606629
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 662 gr
• Formato: Brossura
• Illustration Notes: XII, 336 p. 92 illus., 64 illus. in color.
• Pagine Arabe: 336
• Pagine Romane: xii