TRP Channels in Drug Discovery Volume II

Recent findings have implied a distinct therapeutic potential for drugs targeting Transient Receptor Potential (TRP) channels in a wide variety of diseases, many with no existing satisfactory treatment options. Thus, the TRP superfamily of ion channels has attracted a great deal of well-deserved attention.  TRP Channels in Drug Discovery provides a thorough collection of the most up-to-date reviews and protocols on the subject, coming from top experts in the field.  Volume II presents practical methodologies involving models for disorders of the cardiovascular system, the brain, skin, the metabolic system, as well as colitis, cancer, thermosensation, and musculoskeletal disorders.  Written for the Methods in Pharmacology and Toxicology™ series, this work includes the kind of detailed description and key implementation advice that ensures successful results in the lab.

Part I: TRPs and Cardiovascular Disease

1. TRPs to Cardiovascular Disease

            José C. González-Cobos, Xuexin Zhang, Rajender K. Motiani, Kelly E. Harmon, and Mohamed Trebak

2. Anemic Zebrafish Models of Cardiomyopathy

            Xiaojing Sun and Xiaolei Xu

3. Methods to Study the Effects of TRP Channel Drugs on Vascular Endothelial Cell Function

            Yan Ma, Yung-Wui Tjong, and Xiaoqiang Yao

4. Atherosclerosis Models with Cell-Mediated Calcification

            Beili Zhu

5. Models of Hypertension and Blood Pressure Recording

            Luciana Aparecida Campos and Ovidiu Constantin Baltatu

6. Balloon Injury in Rats as a Model for Studying TRP Channel Contribution to Vascular Smooth Muscle Remodeling

            Wei Zhang and Mohamed Trebak

Part II: TRPs and the Brain

7. TRP Channels in the Brain

            Antonio Reboreda Prieto

8. Investigation of the Possible Role of TRP Channels in Schizophrenia

            Loris A. Chahl

9. Investigating Diseases of Dopaminergic Neurons and Melanocytes Using Zebrafish

            Amanda Decker and Robert Cornell

10. A Practical Guide to Evaluating Anxiety-Related Behavior in Rodents

            Caitlin J. Riebe and Carsten T. Wotjak

11. Rodent Models of Conditioned Fear: Behavioral Measures of Fear and Memory

            Jennifer L. McGuire, Jennifer L. Coyner, and Luke R. Johnson

12. Chick Anxiety-Depression Screening Model

            Stephen W. White and Kenneth J. Sufka

13. A Clinically Relevant Thromboembolic Stroke Model in the Aged Rat

            Ryan C. Turner, Alisa S. Elliott, Jason D. Huber, and Charles L. Rosen

14. Use of Cell-Stretch System to Examine the Characteristics of Mechanosensor Channels: Axonal Growth/Neuroregeneration Studies

            Koji Shibasaki

15. Methods in Neuronal Growth Cone Biology

            Robert J. Gasperini and Lisa Foa

Part III: TRPs and Skin

16. Transient Receptor Potential Channels and Pruritus

            Heike Benecke, Janine Wäring, Tobias Lotts, and Sonja Ständer

17. Skin Sensitivity Studies

            Laurent Misery

18. Hair Follicle Culture

            Michael P. Philpott

Part IV: TRPs and Metabolic Disorders

19. Animal Models for Type 1 Diabetes

            Anish Suri and Matteo Levisetti

20. Type 2 Diabetes Models

            Dorte X. Gram

21. Using Diet to Induce Metabolic Disease in Rodents

            Angela M. Gajda, Michael A. Pellizzon, and Matthew R. Ricci

22. Rodent Models to Evaluate Anti-Obesity Drugs

            Sharon Cheetham and Helen Jackson

Part V: TRPs: Colitis, Cancer, Thermosensation, and Musculoskeletal Disorders

23. Experimental Colitis Models

            Patrick A. Hughes, Stuart M. Brierley, Joel Castro, Andrea M. Harrington, and L. Ashley Blackshaw

24. Tumor Xenograft Models to Study the Role of TRP Channels in Tumorigenesis

            V’yacheslav Lehen’kyi, Sergii Khalimonchyk, Albin Pourtier, Maylis Raphaël, and Natalia Prevarskaya

25. Methods to Study Thermonociception in Rodents

            Kata Bölcskei

26. Methods for the Assessment of Heat Perception in Humans

            Michael F. Crutchlow and Joel D. Greenspan

27. Collagen Antibody-Induced Arthritis (CAIA): A Disease-Relevant Model for Studies of Persistent Joint Pain

            Katalin Sandor, Kutty Selva Nandakumar, Rikard Holmdahl, and Camilla I. Svensson

28. Animal Models of Muscular Dystrophy

            Yuko Iwata and Shigeo Wakabayashi

            Katalin Sandor, Kutty Selva Nandakumar, Rikard Holmdahl, and Camilla I.