Salmonella Methods and Protocols

Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.

1. Luminex^® Multiplex Bead Suspension Arrays for the Detection and Serotyping of Salmonella spp.

Sherry A. Dunbar, Vivette Brown Ritchie, Michaela R. Hoffmeyer, Gunjot S. Rana,  and Hongwei Zhang

2. Quantitative Proteomic Identification of Host Factors Involved in the Salmonella typhimurium Infection Cycle

Dora Kaloyanova, Mijke Vogels, Bas W.M. van Balkom, and J. Bernd Helms

3. Determination of Antimicrobial Resistance in Salmonella spp.

Belgode N. Harish and Godfred A. Menezes

5. A Method to Introduce an Internal Tag Sequence into a Salmonella Chromosomal Gene

Weidong Zhao and Stéphane Méresse

6. Generation and use of Site-directed Chromosomal cyaA’ Translational Fusions in Salmonella enterica

Francisco Ramos-Morales, Elena Cardenal-Muñoz, Mar Cordero-Alba, and Fernando Baisón-Olmo

7. Detection of Antimicrobial (Poly)Peptides with Acid Urea Polyacrylamide Gel Electrophoresis followed by Western Immunoblot

Edith Porter, Erika V. Valore, Rabin Anouseyan, and Nita H. Salzman

8. Detecting non-typhoid Salmonella in Humans by Enzyme-linked

Immunosorbent Assays (ELISAs): Practical and Epidemiological Aspects

Katrin G Kuhn, Hanne-Dorthe Emborg, Karen A Krogfel,  and Kåre Mølbak

9. Study of the Stn Protein in Salmonella; A Regulator of Membrane Composition and Integrity

Masayuki Nakano, Eiki Yamasaki, Joel Moss, Toshiya Hirayama, and Hisao Kurazono

10. Development of a Bacterial Nanoparticle Vaccine

Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho, and Juan M. Irache

11. Direct Attachment of Nanoparticle Cargo to Salmonella typhimurium Membranes Designed for Combination Bacteriotherapy Against Tumors

Robert Kazmierczak, Elizabeth Choe, Jared Sinclair, and Abraham Eisenstark

12. Applications of Microscopy in Salmonella Research

Layla M. Malt, Charlotte A. Perrett, Suzanne Humphrey, and Mark A. Jepson

14. In vitro Modeling of Gallbladder-associated Salmonella spp. Colonization

Geoffrey Gonzalez-Escobedo and J. S. Gunn

15. Salmonella Phages and Prophages – Genomics, Taxonomy,  and Applied Aspects

Andrea I. Moreno Switt, Alexander Sulakvelidze, Martin Wiedmann, Andrew M. Kropinski, David S. Wishart , Cornelis Poppe, and Yongjie Liang