1. Introduction and History of Retinitis Pigmentosa
Hung-Hsi Wang, Nelson Chen, Nan-Kai Wang
2 Etiology of Retinitis Pigmentosa
Mark P. Breazzano, Maeher Grewal, Stephen H. Tsang, and Royce W.S. Chen
3. Clinical Evaluation of Patients with Retinitis Pigmentosa
Sitara H. Hirji
4. Molecular Genetic Testing Approaches for Retinitis Pigmentosa
Megan Soucy, Akemi Joy Tanaka, and Avinash Dharmadhikari
5. Pedigree Analysis of Families and Patients Affected by Retinitis Pigmentosa
Ioana Scherbakova
and Sara D. Ragi
6. Whole Genome Sequencing for Detection of Structural Variants in Patients with Retinitis Pigmentosa
Alexander H. Chai
7. Fundus Imaging Methodologies to Assess RP patients
Jin Kyun Oh, Joseph Ryu, and Stephen H Tsang
8. The Use of Optical Coherence Tomography in Evaluation of Retinitis Pigmentosa
Ha Min Kim, Jin Kyun Oh, Stephen H. Tsang
9. Optical Coherence Tomography Angiography (OCTA) findings in Retinitis Pigmentosa
Vlad Diaconita and Wei Kiong Ngo
10. ERG to Evaluate the Retina in Cases of Retinitis Pigmentosa
Wan-Chun Huang, Pei-Kang Liu, and Nan-Kai Wang
11. Visual Function Tests: Visual Fields
Angela Y. Chang
12. Visual Function Tests
Ritah Chumdermpadetsuk, Sara D. Ragi, and Stephen H. Tsang
13. Visual Acuity as a Measurement of Visual Function
Zachary E. Snow
14. Measure of Visual Function
Sitara H. Hirji
15. Ophthalmic Fluorescein Angiography
Divin R. Baddam, Sara D. Ragi, Stephen H. Tsang, and Wei Kiong Ngo
16. Indocyanine Green Angiography
Divin R. Baddam, Sara D. Ragi, Stephen H. Tsang, and Wei Kiong Ngo
17. Use of the Medmont Dark-Adapted Chromatic Perimeter for Assessing Rod Function in Retinitis Pigmentosa
Yan Nuzbrokh, Sara Ragi, and Stephen H. Tsang
18. Multi-Luminance Mobility Testing Endpoint
Henry W. Zhou
19. Preclinical Models of Retinitis Pigmentosa
Mark D. Travor
20. ERG to evaluate the retina using mouse models
Pei-Kang Liu, Wan-Chun Huang, and Nan-Kai Wang
21. Autofluorescence Imaging to Evaluate Retinal Disease Progression in Rodent
Chia-Hua Cheng and Xuan Cui
22. Large animal models of retinitis pigmentosa in therapy development and preclinical testing
Laurence M Occelli and Simon M Petersen-Jones
23. CRISPR manipulations in stem cell lines
Ya-Ju Chang, Xuan Cui, Sarah R. Levi, Laura A. Jenny, and Stephen H. Tsang
24. Metabolite extraction from RPE cells and retinas related to retinitis pigmentosa
Xuan Cui, Ya-Ju Chang, Laura A. Jenny, Sarah R. Levi, Jianhai Du, and Stephen H. Tsang
25. CRISPR-mediated genome engineering in cell lines
Ya-Ju Chang, Joseph Ryu, Xuan Cui, and Stephen H Tsang
26. CRISPR off-targeting
Christine L. Xu, Merry Zhechao Ruan, Sara D. Ragi, and Stephen H. Tsang
27. CRISPR manipulations in stem cell lines
Ya-Ju Chang, Xuan Cui, Sara D. Ragi, and Stephen H. Tsang
28. Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV-Mediated Gene Delivery
Amy Tso, Bruna Lopes da Costa, Laura A. Jenny, Sarah R. Levi, Alexandra Fehnel, Sara D. Ragi, Yao Li, and Peter M.J. Quinn
29. Culture of Human Retinal Explants for Ex Vivo Assessment of AAV Gene Delivery
Wen-Hsuan Wu, Amy Tso, Mark P. Breazzano, Sarah R. Levi, Laura A. Jenny, Stephen H. Tsang, and Peter M.J. Quinn
30. Prime Editing for the Installation and Correction of Mutations causing Inherited Retinal Disease: A Brief Methodology
Yi-Ting Tsai, Bruna Lopes da Costa, Nicholas D. Nolan, Salvatore M. Caruso, Laura A. Jenny, Sarah R. Levi, Stephen H. Tsang, and Peter M.J. Quinn
31. Large scale single-nucleus RNA sequencing compatible with complex archived samples
Chao-Yu Liu and Hsu-Hsin Chen
32. Vector Shedding and Immunogenicity Sampling in AAV-based Gene Therapy for Retinitis Pigmentosa Patients
Ahra Cho
33. Current Management Options for Patients with Retinitis Pigmentosa
Abinaya Thenappan
34. Challenges of Treatment Methodologies and the Future of Gene Therapy and Stem Cell Therapy to Treat Retinitis Pigmentosa
Angela Y. Chang
35. Ocular Injection Techniques for Retinitis Pigmentosa: Intravitreal, Subretinal, and Suprachoroidal
Ioana Scherbakova, Sara D. Ragi, and Tarun Sharma
36. Surgical Approach with Pars Plana Vitrectomy for Subretinal Gene Therapy
Mark P. Breazzano, Sean A. Batson, Stephen H. Tsang, and Royce W.S. Chen
