Neutrophil Methods and Protocols

Neutrophils: An Overview.- The Role of Neutrophils in the Immune System.- Neutrophil Isolation and Subcellular Fractionation.- Isolation of Human Neutrophils From Venous Blood.- Neutrophil Isolation From Nonhuman Species.- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers.- Biochemistry, Biology, and Signal Transduction of Neutrophils.- Rac and Rap GTPase Activation Assays.- Measurement of Phospholipid Metabolism in Intact Neutrophils.- Analysis of Protein Phosphorylation in Human Neutrophils.- Mitogen-Activated Protein Kinase Assays.- Transduction of Proteins into Intact Neutrophils.- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils.- Analysis of Electrophysiological Properties and Responses of Neutrophils.- Analysis of Neutrophil Apoptosis.- Neutrophil Adhesion and Chemotaxis.- Optical and Fluorescence Detection of Neutrophil Integrin Activation.- Analysis of Neutrophil Polarization and Chemotaxis.- Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils.- Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow.- Model Systems to Investigate Neutrophil Adhesion and Chemotaxis.- Neutrophil Phagocytosis and Bactericidal Activity.- Immunofluorescence and Confocal Microscopy of Neutrophils.- Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy.- Analysis of Neutrophil Membrane Traffic During Phagocytosis.- Analysis of Neutrophil Bactericidal Activity.- A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions.- Nadph Oxidase and Production of Reactive Oxygen Species.- Measurement of Respiratory Burst Products Generated by Professional Phagocytes.- The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function.- Cell-Free Assays.- Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics.- Analysis of Neutrophil Gene Expression and Transcription Factors.- Genome-Scale Transcript Analyses in Human Neutrophils.- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR.- Detection of Intact Transcription Factors in Human Neutrophils.- Neutrophil Defects and Diagnosis.- Disorders of Neutrophil Function.- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders.- Diagnostic Assays for Myeloperoxidase Deficiency.