Part I: Noble Metal Nanoparticles: Synthesis and Applications
1. Procedures for the Synthesis and Capping of Metal Nanoparticles
Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, and Miguel José-Yacamán
2. Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications
Yeon-Su Park
3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis
Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, and Sangiliyandi Gurunathan
4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays
Julian Bailes, Samantha Mayoss, Phil Teale, and Mikhail Soloviev
5. Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues
Sadaki Yokota
6. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics
Joao Conde, Goncalo Doria, Jesus M. de la Fuente, and Pedro Viana Baptista
7. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins
Julian Bailes, Sara Gazi, Rositsa Ivanova, and Mikhail Soloviev
Part II: Semiconductor Nanoparticles: Synthesis and Applications
8. Synthesis of Semiconductor Nanoparticles
Xianfeng Chen and Peter J. Dobson
9. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature
Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, and Yoshinobu Baba
10. Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods
Ana Sofia Miguel, Christopher Maycock, and Abel Oliva
11. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing
Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, and Paris A. Skourides
12. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis
Babu R. Prasad, Enda O’ Connell, Terry J. Smith, Valérie A. Gérard, Yurii K. Gun’ko, and Yury Rochev
13. The Use of Quantum Dots for Immunochemistry Applications
Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, and Carme Nogués
14. Quantum Dots for Labelling Live Cells
Yun Chen
15. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots
Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, and Barbara J. Muller-Borer
16. The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
Julian Bailes and Mikhail Soloviev
Part III: Magnetic Nanoparticles: Synthesis and Applications
17. Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging
Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen, and Ning Gu
18. Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging
Jasmin, Ana Luiza Machado Torres, Linda Jelicks, Antonio Carlos Campos de Carvalho, David C. Spray, and Rosalia Mendez-Otero
19. Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry
Maik Liebl and Dietmar Eberbeck
20. Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants
Alejandro Pérez-de-Luque, Zuny Cifuentes, Clara Marquina, Jesús M. de la Fuente, and M. Ricardo Ibarra
Part IV: Organic, Polymeric, Biomimetic, and Biocompatible Nanoparticles and Their Applications
21. Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”)
Valérie A. Gérard, Yurii K. Gun’ko, Babu R. Prasad, and Yury Rochev
22. Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery
Ana Maria Carmona-Ribeiro
23. Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery
Hao-Ying Li and Fan Zhang
24. Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases
Neeraj Vij
25. Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency
Panayotis Pantazis, Konstantinos Dimas, James H. Wyche, Shrikant Anant, Courtney W. Houchen, Jayanth Panyam, and Rama P. Ramanujam
26. Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications
Rajesh Saini, Jaya Bajpai, and Anil K. Bajpai
27. Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection
Fan Zhang and Hao-Ying Li
28. Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications
Mirian A.F. Hayashi, Eduardo B. Oliveira, Irina Kerkis, and Richard L. Karpel
29. Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins
Raul Machado, Paulo C. Bessa, Rui L. Reis, José Carlos Rodriguez-Cabello, and Margarida Casal
30. Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles
Diksha and Indrajit Roy
31. Extraction of Organic Nanoparticles from Plants
Scott C. Lenaghan, Qin Zhu, Lijin Xia, and Mingjun Zhang
Part V: Nano-Toxicity and Nano-Safety
32. Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay
Amanda M. Schrand, Jonathan B. Lin, and Saber M. Hussain
33. Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression
Min Ding, Linda Bowman, and Vincent Castranova
34. Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells
Linda Bowman, Vincent Castranova, and Min Ding
35. Assessment of Toxicity of Nanoparticles Using Insects as Biological Models
Yan Zhou, Aracely Rocha, Carlos J. Sanchez, and Hong Liang
36. Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues
Ana Raquel Santos, Ana Sofia Miguel, Pedro Fevereiro, and Abel Oliva
Part VI: Nanoparticles Characterization
37. Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles
Arturo Ponce, Sergio Mejía-Rosales, and Miguel José–Yacamán
38. Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction
Ladan Parhamifar and S. Moein Moghimi
39. Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells
Robert Martin Zucker and Kaitlin Marie Daniel
40. Detection of TiO2 Nanoparticles in Cells by Flow Cytometry
Robert Martin Zucker and Kaitlin Marie Daniel
41. NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions
Matthew Wright
42. Relevance of Two-Dimensiona
