DNA Sequencing Protocols

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

to DNA Sequencing.- The Universal Primers and the Shotgun DNA Sequencing Method.- M13 Sequencing.- Primer Design and Primer-Directed Sequencing.- Direct Sequencing of DNA Produced in a Polymerase Chain Reaction.- Solid Phase Fluorescent Sequencing of the CFTR Gene.- Shotgun DNA Sequencing.- Cycle Sequencing.- Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers.- Automated Fluorescent DNA Sequencing on the ABI PRISM 377.- Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer.- Fluorescent Sequencing Protocols for the ALF.- Fluorescent Sequencing for Heterozygote Mutation Detection.- Sequence Databases and the Internet.- DNA Sequencing by Capillary Array Electrophoresis.