Clinical and Forensic Applications of Capillary Electrophoresis

Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance.
Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.

Section I. Introduction Capillary Electrophoresis: General Overview and Applications in the Clinical Laboratory Manjiri Lele, Subodh M. Lele, John R. Petersen, and Amin A. Mohammad Basic Principles and Modes of Capillary Electrophoresis Harry Whatley Section II. Protein Electrophoresis Capillary Coatings for Protein Analysis Kannan Srinivasan, Chris Pohl, and Nebojsa Avdalovic Clinical Serum Protein Capillary Zone Electrophoresis Carl R. Jolliff Serum and Urine Paraprotein Capillary Electrophoresis Carl R. Jolliff Cerebrospinal Fluid Protein Electrophoresis John R. Petersen and Amin A. Mohammad Lipoprotein Analysis Rainer Lehmann Clinical Analysis of Structural Hemoglobin Variants and Hb A1c by Capillary Isoelectric Focusing James M. Hempe, Alfonso Vargas, and Randall D. Craver Section III. Metabolic Diseases Amino Acid Analysis, Mary Kathryn Linde. Organic Acids Kern L. Nuttall and Norberto A. Guzman Steroids, Cecilla Youh Amin A. Mohammad, and John R. Petersen Section IV. Immunoassay Capillary Electrophoresis Based Immunoassay Bode Adesoji, Amin A. Mohammad, and John R. Petersen Section V. Molecular Diagnostics Quantitation of Viral Load Jill M. Kolesar The Application of Capillary Electrophoresis in the Analysis of PCR Products used in Forensic DNA Typing Bruce R. McCord and John M. Butler Capillary Electrophoresis with Electrospray Ionization Mass Spectrometry Samir Cherkaoui Section VI. Mass Spectrometry, Therapeutic Drug Monitoring, and Toxicology Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and Proteins Stephen Naylor and Andy J. Tomlinson Serum Drug Monitoring by Capillary Electrophoresis Zack K. Shihabi Applications of Capillary Zone Electrophoresis in the Analysis of Metal Ions of Clinical Significance Lokinendi V. Rao, John R. Petersen, Amin A. Mohammad, and Anthony O. Okorodudu Clinical and Forensic Drug Toxicology: Analysis of Illicit and Abused Drugs in Urine by Capillary Electrophoresis Wolfgang Thormann and Jitka Caslavska Screening Biological Specimens for Drugs of Forensic Significance John C. Hudson, Murray J. Malcolm, and Mauro Golin