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In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.
1. Nanoparticle-GFP “Chemical Nose” Sensor for Cancer Cells Identification
Daniel F. Moyano and Vincent M. Rotello
2. A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism using a DNA Nanosensor
Sunaina Surana and Yamuna Krishnan
3. A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells using a Fluorescent Dye
Syed K. Sohaebuddin and Liping Tang
4. Gold Nanoparticle as a Marker for Precise Localization of Nano-objects within Intracellular Sub-Domains
Valeriy Lukyanenko and Vadim Salnikov
5. Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation
Ari Nowacek, Irena Kadiu, JoEllyn McMillan and Howard E. Gendelman
6. Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments
Ari Nowacek, Irena Kadiu, JoEllyn McMillan and Howard E. Gendelman
7. Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains
Valeriy Lukyanenko
8. A Method to Encapsulate Molecular Cargo within DNA Icosahedra
Dhiraj Bhatia, Saikat Chakraborty, Shabana Mehtab and Yamuna Krishnan
9. Delivery of Plasmid DNA to Mammalian Cells using Polymer-Gold Nanorod Assemblies
James Ramos, Huang-Chiao Huang, and Kaushal Rege
10. Lipophilic Formulated Gold Porphyrin Nanoparticles for Chemotherapy
Puiyan Lee and Kenneth KY Wong
11. Mitochondria-specific Nano-Emulsified Therapy for Myocardial Protection against Doxorubicin-induced Cardiotoxicity
A.Faulk, V. Weissig and Tamer Elbayoumi
12. Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events
Indriati Pfeiffer and Michael Zäch
13. Characterization of Nanoparticle – Lipid Membrane Interactions using QCM-D
Rickard Frost and Sofia Svedhem
14. Single-Cell Nanosurgery
Maxwell B. Zeigler and Daniel T. Chiu
15. Single Quantum Dot Imaging in Living Cells
Jerry C. Chang and Sandra J. Rosenthal
16. Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging
Sijie Chen, Jacky W. Y. Lam, and Ben Zhong Tang
17. Monitoring the Degradation of Reduction Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry
Constantin Hozsa, Miriam Breunig, and Achim Göpferich
18. Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces
Yaron R. Silberberg and Andrew E. Pelling
19. Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery
Paul D. R. Dyer, Arun K. Kotha, Marie W. Pettit, Simon C. W. Richardson
20. Real-Time Particle Tracking for Studying Intracellular Trafficking of Pharmaceutical Nanocarriers
Feiran Huang, Erin Watson, Christopher Dempsey, and Junghae Suh
21. Interactions of Nanoparticles with Proteins -Determination of Equilibrium Constants
Lennart Treuel, Marcelina Malissek
22. Tracing the Endocytic Pathways and Trafficking Kinetics of Cell Signaling Receptors using Single QD Nanoparticles
Katye M. Fichter and Tania Q. Vu
23. Cellular Internalization of Quantum DotsYue-Wern Huang, Han-Jung Lee, Betty Revon Liu, Huey-Jenn Chiang, Chi-Heng Wu
24. Electrochemical Scanning Tunneling Microscopy and Spectroscopy for Single Molecule Investigation
Andrea Alessandrini & Paolo Facci
25. Intracellular Delivery of Biologically Active Proteins with Peptide-based Carriers
S.L. Lo and S. Wang
26. Lipo-oligoarginine-based Intracellular Delivery
Jae Sam Lee and Ching-Hsuan Tung
27. Fluorescent Spherical Monodisperse Silica Core-shell Nanoparticles with a Protein-binding Biofunctional Shell
Achim Weber, Marion Herz and Günter E.M. Tovar
28. Direct Quantification of PTD Transduction Using Real-Time Monitoring
Mi-Sook Lee and Song Her
29. Genotoxic Assessment of Carbon Nanotubes
O. Neškovic, G. Joksic, A. Valenta Šobot, J. Cveticanin, Dj. Trpkov, A. Leskovac and S. Petrovic
30. Separation Science: Principles and Applications for the Analysis of Bionanoparticles by Asymmetrical Flow Field-Flow Fractionation (AF4)
Alexandre Moquin, Françoise M. Winnik, and Dusica Maysinger
31. Polymersomes-mediated Delivery of Fluorescent Probes for Targeted and Long Term Imaging in Live Cell Microscopy
Irene Canton and Giuseppe Battaglia
32. Protocol for the Preparation of Stimuli-responsive Gold Nanoparticles Capped with Elastin-based Pentapeptides
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