Shiga Toxin-Producing E. coli

TRAMA

This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing E. coli  (STEC) and Shiga toxins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Shiga Toxin-Producing E. coli: Methods and Protocols aims to be a valuable resource for clinicians, epidemiologists, and researchers interested in STEC pathogenesis.

SOMMARIO

1. Integrated Approach for the Diagnosis of Shiga Toxin-producing Escherichia coli Infections in Humans Stefano Morabito, Fabio Minelli and Rosangela Tozzoli   2. Shiga Toxin-producing E. coli in Animals – Detection, Characterisation, and Virulence Assessment Stefanie A. Barth, Rolf Bauerfeind, Christian Berens, and Christian Menge   3. Identification of Shiga Toxin-producing Escherichia coli Outbreaks using Whole Genome Sequencing Stefan Bletz, Alexander Mellmann, and Barbara Middendorf-Bauchart   4. Predicting Host Association for Shiga Toxin-producing E. coli serogroups by Machine Learning Nadejda Lupolova, Antonia Chalka, and David L. Gally   5. Isolation and Characterization of Shiga Toxin Bacteriophages Lorena Rodríguez-Rubio and Maite Muniesa   6. Lambda Red Mediated Recombination in Shiga toxin-producing Escherichia coli  Kenneth G Campellone and Alyssa M Coulter   7. Functional Analysis of Shiga Toxin-producing Escherichia coli Biofilm Components in Plant Leaves Nicola J. Holden, Kathryn M. Wright, Jacqueline Marshall, and Ashleigh Holmes   8. Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-producing Escherichia coli Outer Membrane Vesicles Martina Bielaszewska, Lilo Greune, Andreas Bauwens, Petra Dersch,  Alexander Mellmann, and Christian Rüter   9. Isolation and Characterization of Shiga Toxin-Associated Microvesicles Annie Willysson, Anne-lie Ståhl, and  Diana Karpman   10. ­­Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Receptors Johanna Detzner, Gottfried Pohlentz, and Johannes Müthing   11. Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-producing Escherichia coli to Epithelial Cells David Ruano-Gallego and Luis Ángel Fernández   12. Determining Shiga Toxin-producing Escherichia coli Interactions with Human Intestinal Epithelium in a Microaerobic Vertical Diffusion Chamber Conor J. McGrath and Stephanie Schüller 13. Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host Interactions Karol Dokladny, Julie G. In, James Kaper, and Olga Kovbasnjuk   14. Use of the Dynamic TIM-1 Model for an In-depth understanding of the Survival and Virulence Gene Expression of Shiga toxin-producing Escherichia coli in the Human Stomach and Small Intestine Ophélie Uriot, Sandrine Chalancon, Carine Mazal, Lucie Etienne-Mesmin, Sylvain Denis, and Stéphanie Blanquet-Diot   15. Measuring Effector-mediated Modulation of Inflammatory Responses to Infection with Enteropathogenic and Shiga toxin-producing E. coli Georgina L. Pollock, Cristina Giogha, and Elizabeth L. Hartland   16. Interaction of Bovine Lymphocytes with Products of Shiga toxin-Producing Escherichia coli Andrew G. Bease, Robin L. Cassady-Cain, and Mark P. Stevens   17. Infection of Immunocompetent Conventional Mice with Shiga toxin-producing E. coli:  The DSS+STEC Model Gregory Hall, Shinichiro Kurosawa, and D. J. Stearns-Kurosawa   18. Infant Rabbit Model for Studying Shiga Toxin-producing Escherichia coli Jennifer M. Ritchie   19. Citrobacter rodentium lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-producing E. coli Infection Laurice J. Flowers, Shenglan Hu , Anishma Shrestha, Amanda J. Martinot, John M. Leong, and Marcia S. Osburne   20. Overview of the Effect of Citrobacter rodentium Infection On Host Metabolism And The Microbiota Eve G. D. Hopkins and Gad Frankel  

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071613382
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 1012 gr
• Formato: Copertina rigida
• Illustration Notes: XI, 421 p. 78 illus., 52 illus. in color.
• Pagine Arabe: 421
• Pagine Romane: xi