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This volume provides a comprehensive outline of the current methods used to detect, characterize, and investigate Shiga toxin-producing E. coli (STEC) and Shiga toxins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Shiga Toxin-Producing E. coli: Methods and Protocols aims to be a valuable resource for clinicians, epidemiologists, and researchers interested in STEC pathogenesis.
1. Integrated Approach for the Diagnosis of Shiga Toxin-producing Escherichia coli Infections in Humans
Stefano Morabito, Fabio Minelli and Rosangela Tozzoli
2. Shiga Toxin-producing E. coli in Animals – Detection, Characterisation, and Virulence Assessment
Stefanie A. Barth, Rolf Bauerfeind, Christian Berens, and Christian Menge
3. Identification of Shiga Toxin-producing Escherichia coli Outbreaks using Whole Genome Sequencing
Stefan Bletz, Alexander Mellmann, and Barbara Middendorf-Bauchart
4. Predicting Host Association for Shiga Toxin-producing E. coli serogroups by Machine Learning
Nadejda Lupolova, Antonia Chalka, and David L. Gally
5. Isolation and Characterization of Shiga Toxin Bacteriophages
Lorena Rodríguez-Rubio and Maite Muniesa
6. Lambda Red Mediated Recombination in Shiga toxin-producing Escherichia coli
Kenneth G Campellone and Alyssa M Coulter
7. Functional Analysis of Shiga Toxin-producing Escherichia coli Biofilm Components in Plant Leaves
Nicola J. Holden, Kathryn M. Wright, Jacqueline Marshall, and Ashleigh Holmes
8. Virulence Factor Cargo and Host Cell Interactions of Shiga Toxin-producing Escherichia coli Outer Membrane Vesicles
Martina Bielaszewska, Lilo Greune, Andreas Bauwens, Petra Dersch, Alexander Mellmann, and Christian Rüter
9. Isolation and Characterization of Shiga Toxin-Associated Microvesicles
Annie Willysson, Anne-lie Ståhl, and Diana Karpman
10. Thin-Layer Chromatography in Structure and Recognition Studies of Shiga Toxin Glycosphingolipid Receptors
Johanna Detzner, Gottfried Pohlentz, and Johannes Müthing
11. Identification of Nanobodies Blocking Intimate Adherence of Shiga Toxin-producing Escherichia coli to Epithelial Cells
David Ruano-Gallego and Luis Ángel Fernández
12. Determining Shiga Toxin-producing Escherichia coli Interactions with Human Intestinal Epithelium in a Microaerobic Vertical Diffusion Chamber
Conor J. McGrath and Stephanie Schüller
13. Human Epithelial Stem Cell-Derived Colonoid Monolayers as a Model to Study Shiga Toxin-Producing Escherichia coli-Host InteractionsKarol Dokladny, Julie G. In, James Kaper, and Olga Kovbasnjuk
14. Use of the Dynamic TIM-1 Model for an In-depth understanding of the Survival and Virulence Gene Expression of Shiga toxin-producing Escherichia coli in the Human Stomach and Small Intestine
Ophélie Uriot, Sandrine Chalancon, Carine Mazal, Lucie Etienne-Mesmin, Sylvain Denis, and Stéphanie Blanquet-Diot
15. Measuring Effector-mediated Modulation of Inflammatory Responses to Infection with Enteropathogenic and Shiga toxin-producing E. coli
Georgina L. Pollock, Cristina Giogha, and Elizabeth L. Hartland
16. Interaction of Bovine Lymphocytes with Products of Shiga toxin-Producing Escherichia coli
Andrew G. Bease, Robin L. Cassady-Cain, and Mark P. Stevens
17. Infection of Immunocompetent Conventional Mice with Shiga toxin-producing E. coli: The DSS+STEC Model
Gregory Hall, Shinichiro Kurosawa, and D. J. Stearns-Kurosawa
18. Infant Rabbit Model for Studying Shiga Toxin-producing Escherichia coli
Jennifer M. Ritchie
19. Citrobacter rodentium lysogenized with a Shiga Toxin-Producing Phage: A Murine Model for Shiga Toxin-producing E. coli Infection
Laurice J. Flowers, Shenglan Hu , Anishma Shrestha, Amanda J. Martinot, John M. Leong, and Marcia S. Osburne
20. Overview of the Effect of Citrobacter rodentium Infection On Host Metabolism And The Microbiota
Eve G. D. Hopkins and Gad Frankel
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