Experimental Models of Cardiovascular Diseases Methods and Protocols

This book provides methodological information on establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, leading to novel discoveries in cardiac biology,  and the development of effective therapeutic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Experimental Models of Cardiovascular Disease: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Overview

1.                  Experimental Models of Cardiovascular Diseases - Overview -

Part II :In Silico Models

2.                  An Introduction to Computational Modeling of Cardiac Electrophysiology and Arrhythmogenicity

Joshua Mayourian, Eric A. Sobie, and Kevin D. Costa

Part III: In Vitro Models

3.                  Isolation of Atrial and Ventricular Cardiomyocytes for In Vitro Studies

Jelena Plackic and Jens Kockskämper

4.                  Cardiomyocyte Differentiation from mouse Embryonic Stem Cells 

Adam T Lynch, Silvia Mazzotta, and Stefan Hoppler

5.                  Cardiomyocyte Differentiation from Human Embryonic Stem Cells 

Silvia Mazzotta, Adam T Lynch, and Stefan Hoppler

6.                  Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes using Embryoid Bodies

Takeshi Hatani1, Kenji Miki, and Yoshinori Yoshida

7.                  Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro

Przemek A. Gorski, Changwon Kho, and Jae Gyun Oh

Part IV: Ex Vivo Models

8.                  Langendorff Perfusion Method as an Ex Vivo Model to Evaluate Heart Function in Rats

Makino Watanabe and Takao Okada

9.                  Methods for the Preparation of an Excised, Cross-circulated Rat Heart

Koji Obata and Miyako Takaki

10.              Optical Action Potential Mapping in Acute Models of Ischemia-reperfusion Injury: Probing the Arrhythmogenic Role of the Mitochondrial Translocator Protein

Zeki Ilkan, Benjamin Strauss, Chiara Campana, and Fadi G. Akar

11.              Cardiac Tissue Engineering Models of Inherited and Acquired Cardiomyopathies

Irene C. Turnbull, Joshua Mayourian, Jack F. Murphy, Francesca Stillitano,

Delaine K. Ceholski, and Kevin D. Costa

12.              Badimon Perfusion Chamber: An Ex Vivo Model of Thrombosis

M. Urooj Zafar, Carlos G. Santos-Gallego, Lina Badimon, and Juan J. Badimon,

Part V: Small Animal Models

13.              Ischemic Model of Heart Failure in Rats and Mice

Jiqiu Chen, Delaine K. Ceholski, Irene C. Turnbull, Lifan Liang, and Roger J. Hajjar

14.              Conventional Method of Transverse Aortic Constriction in Mice

Jimeen Yoo, Vadim Chepurko, Roger J. Hajjar, and Dongtak Jeong

15.              Characterization of the Differential Progression of Left Ventricular Remodeling in a Rat Model of Pressure Overload Induced Heart Failure. Does Clip Size Matter?

Antoine H. Chaanine and Roger J. Hajjar

16.              Isoproterenol-induced Heart Failure Mouse Model Using Osmotic Pump Implantation

17.              Rat model of Cardiotoxic Drug Induced Cardiomyopathy

Takehiro Nakahara, Takashi Tanimoto, Artiom D. Petrov, Kiyotake Ishikawa,  H. William Strauss, and Jagat Narula

18.              Pulmonary Artery Hypertension Model in Rats by Monocrotaline Administration

Carlos Bueno-Beti, Yassine Sassi, Roger J. Hajjar, and Lahouaria Hadri

19.              The Sugen 5416/Hypoxia Mouse Model of Pulmonary Arterial Hypertension

Carlos Bueno-Beti, Lahouaria Hadri, Roger J. Hajjar, and Yassine Sassi

20.              Mouse Model of Wire Injury-Induced Vascular Remodeling

Aya Nomura-Kitabayashi and Jason C. Kovacic

21.              The Mouse Aortocaval Fistula Model with Intraluminal Drug Delivery

Toshihiko Isaji and Alan Dardik

Part VI: Large Animal Models

22.              A Pig Model Of Myocardial Infarction: Catheter Based Approaches

Olympia Bikou, Shin Watanabe, Roger J. Hajjar, and Kiyotake Ishikawa

Dae-Hee Kim, Morris Brittan, J. Luis Guerrero, Suzanne M. Sullivan, Judy Hung, and Robert A. Levine

24.              Canine Model of Pacing-induced Heart Failure

Jeffery C. Powers and Fabio Recchia

25.              Swine Model of Mitral Regurgitation Induced Heart Failure

26.              Pig Model of Increased Cardiac Afterload Induced by Ascending Aortic Banding

Olympia Bikou, Satoshi Miyashita, and Kiyotake Ishikawa

27.              Large Porcine Model of Profound Acute Ischemic Cardiogenic Shock

Ole K. Møller-Helgestad, Hanne B. Ravn, and Jacob E. Møller

28.              Chronic Pulmonary Artery Embolization Models in Large Animals

Jaume Aguero, Nadjib Hammoudi, Olympia Bikou, Kenneth M. Fish, Iratxe Zarragoikoetxea, Roger J. Hajjar, and Kiyotake Ishikawa

29.              Modeling Pulmonary Hypertension: A Pig Model Of Post-capillary Pulmonary Hypertension

Olympia Bikou, Kiyotake Ishikawa, Kenneth M. Fish, Iratxe Zarragoikoetxea, Roger J. Hajjar, and Jaume Aguero

30.              Development and Multiparametric Evaluation of Experimental Atherosclerosis in Ra