Cardiac Gene Therapy Methods and Protocols

This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. 

Updates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy.- Tough Decoy-Mediated Cardiac Gene Suppression.- Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes using miRcombo.- CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC Derived Cardiomyocytes to Probe Ca²⁺ Signaling Aberrancies of CPVT Arrhythmogenesis.- Enhancing Cardiomyocyte Transcription using In Vivo CRISPR/Cas9 Systems.- AAV Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model.- Optimization of Synthesis of Modified mRNA.- Design and Production of Heart Chamber-Specific AAV9 Vectors.- Generation of Atrial-Specific Construct using Sarcolipin Promoter Associated CRM4 Enhancer.- Cardiac Targeted Adeno-Associated Virus Injection in Rats.- Cardiac Gene Delivery in Large Animal Models-Antegrade Techniques.- Locked Nucleic Acid AntimiR Therapy for the Heart.- Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection.- Endocardial Gene Delivery using NOGA Catheter System.- Surgical Methods for Cardiac Gene Delivery in Large Animals.- Atrial Gene Painting in Large Animal Model of Atrial Fibrillation.- Stent-Based Gene Delivery for Coronary Disease.- Selective Anti-AAV Antibody Depletion by Hemapheresis and Immunoadsorption.- Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy.- Intra-Airway Gene Delivery for Pulmonary Hypertension in Rodent Models.- Endo-Bronchial Gene Delivery for Pulmonary Hypertension in a Large Animal Model.- Cell-Based Determination of Neutralizing Antibodies against Adeno-Associated Virus in Cardiac Gene Therapy.- Left Ventricular Pressure Volume Assessment Using Carotid Artery Access in the Rat.- Assessing the Effect of Cardiac Gene Therapy using Catheter-Based Pressure-Volume Measurement in Large Animals.- ELIspot Assay for Gene Therapy in Large Animal Studies.- Assessing Recombinant AAV Shedding after Cardiac Gene Therapy.