Xylem Methods and Protocols

This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue.  Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.

Experimental and Theoretical Methods to Investigate Vascular Development in Plants.- Strigolactone-Mediated Stimulation of Secondary Xylem Proliferation in Stems.- Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.- Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis.- Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.- VND6-Induced Xylem Cell Differentiation in Arabidopsis Cell Cultures.- Live Imaging of Developing Xylem in Planta.- Immunolocalization in Secondary Xylem of Populus.- Monitoring Vascular Regeneration and Xylem Connectivity In Arabidopsis Thaliana.- Vascular Morphodynamics during Secondary Growth.- Improved Mps-PI Staining for Xylem Characterization.- Chemical Imaging of Xylem by Raman Microspectroscopy.- Using Cellprofiler to Analyse and Quantify Vascular Morphology .- Lignin Analysis by HPLC and FTIR.- Carbohydrate Composition Analysis in Xylem.- Structural Analysis of Cell Wall Polysaccharides Using PACE.- Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy.