Type 2 Immunity Methods and Protocols

A Fungal Protease Model To Interrogate Allergic Lung Immunity.- Use of the Litomosoides sigmodontis Infection Model of Filariasis to study Type 2 Immunity.- Production of Hymenolepis diminuta in the Laboratory: An Old Research Tool With New Clinical Applications.- A Mouse Model of Peanut Allergy Induced By Sensitization through the Gastrointestinal Tract.- Induction and Characterization of the Allergic Eye Disease Mouse Model.- Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung.- In vitro and In vivo IgE/Antigen Mediated Mast Cell Activation.- The use of Human and Mouse Mast Cell and Basophil Cultures to Assess type-2 Inflammation.- Isolation and Identification of Group 2 Innate Lymphoid Cells in Settings of Type 2 Inflammation.- Determination of the Fate and Function of Innate Lymphoid Cells Following Adoptive Transfer of Innate Lymphoid Cell Precursors.- Characterization of Thymic Development of Natural Killer T Cell Subsets by Multiparameter Flow Cytometry.- Characterization of Mouse gd T Cell Subsets in the Setting of type-2 Immunity.- The Identification of Allergen-derived T cell Epitopes.- Generation of Allergen-specific Tetramers for a Murine Model of Airway Inflammation.- The Generation and Use of Allergen-specific TCR Transgenic Animals.- Using Cytokine Reporter Mice to Visualize Type-2 immunity in vivo.- Live Imaging of IL-4 expressing T Follicular Helper Cells in Explanted Lymph Nodes.- Imaging Precision Cut Lung Slices to Visualize Leukocyte Localization and Trafficking.- Study of IgE-producing B cells using the Verigem Fluorescent Reporter Mouse.- Chromatin Preparation from Murine Eosinophils for Genome-wide Analyses.- A Sensitive and Integrated Approach to Profile Messenger RNA from Samples with Low Cell Numbers.- An Integrated and Semi-automated Micro-scaled Approach to Profile Cis-Regulatory Elements by Histone Modification ChIP-Seq for Large-scale Epigenetic Studies.- Library preparation for ATAC-sequencing of Mouse CD4+ T Cells Isolated from the Lung and Lymph Nodes after Helminth Infection.- Identification of Functionally-relevant microRNAs in the Regulation of Allergic Inflammation.- The Use of Biodegradable Nanoparticles for Tolerogenic Therapy of Allergic Inflammation.- Assessing the Mouse Intestinal Microbiota in Settings of type-2 Immune Responses.- The use of CRISPR-Cas9 Technology to Reveal Important Aspects of Human Airway Biology.- A Consistent Method to Identify and Isolate Mononuclear Phagocytes from Human Lung and Lymph Nodes.- Organoid Cultures for Assessing Intestinal Epithelial Differentiation and Function in Response to Type-2 Inflammation.- Utilization of Air-liquid Interface Cultures as an in vitro Model to Assess Primary Airway Epithelial Cell Responses to the Type 2 Cytokine Interleukin 13.