Tick-Borne Viruses Methods and Protocols

This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed protocols on how to conduct in-depth research on TBVs; enhance understanding of the virus’ biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory protocols, and includes troubleshooting tips to help avoid common issues.

Cutting-edge and thorough, Tick-Borne Viruses: Methods and Protocols is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs.

 Tick Cell Culture Methods.- Virus Isolation and Culture from Field Collected Ticks: In Vitro.- Virus Isolation from Field-Collected Ticks: In Vivo.- Generation of Ticks Infected with Tick-Borne Encephalitis Virus.- Generation of Virus Infected Ticks: Synchronous Infection and Feeding on Viremic Animals.- Human Skin Ex-Vivo Culture for Tick-Borne Virus Infection Studies.- Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection.- Isolation and Quantitation of Dabie bandavirus.- Immunofluorescence Assays to Visualize Tick-Borne Flaviviruses in Mouse Skin or Brain.- Immunohistochemical Assays to Visualize Tick-Borne Flaviviruses at the Tick Feeding Site.- RNA In-Situ Hybridization to Visualize Tick-Borne Viruses.- Quantification of Tick-Borne Flaviviruses Using Focus Forming Assays.- Quantification of Neutralizing Antibodies to Tick-Borne Viruses using Focus Neutralization Assays.- Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus.- Exploring Tick-Borne Encephalitis Virus-Host Cell Interactions using Electron Tomography: Methodologies and Protocols.