The Surfaceome Methods and Protocols

This volume explores a collection of methodologies used to study the complexities of biological membranes and the associated surfaceome. The chapters in this book aim to facilitate the adoption of innovative methods to help researchers advance their understanding of the biology of membranes and their role in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.

Comprehensive and cutting-edge, The Surfaceome: Methods and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this important and developing field.

Introduction: Methods for Studying the Plasma Membrane and the Surfaceome.- Optimized Biotinylated Peptide Detection Method for Characterizing the Cell Surface Proteome.- Proximity Labeling of Cell Surface Proteins via Cell Surface Remodeling.- BioID-Based Proximity Mapping of Transmembrane Proteins in Human Airway Cell Models.- Aminooxy Biotin-Based Characterization of the Surfaceome of Chondrogenic Cells.- Isolation of the Surfaceome from Primary Human Epidermal Melanocytes using Glycocapture and Shotgun Proteomics.- Dissecting the Heterogeneity of Senescent Cells through CITE-seq Integration.- Metal Organic Layers Based Sample Preparation Method for Membrane Proteome Analysis.- Updated Methods for Elucidating Bacterial Surfaceomes.- Functional Characterization of Ion Channels in Planar Lipid Bilayers.- Direct Assessment of Temperature Sensitivity of the TRPM2 Channel with the Patch Clamp Technique.- Measuring Molecular Interactions with Subcellular Resolution - Single Cell FRET Using the Quantitative Three-Filterset (Intensity-Based) Approach.- Quantifying the Electrophysiological Properties of Cells Using Dielectrophoresis.- Biophysical Profiling of Protein Corona on Red Blood Cell-Derived Extracellular Vesicles (REVs): Linear Dichroism and Microfluidic Resistive Pulse Sensing Separates Surface Clearing from Vesicle Disruption.- ATR-Infrared Spectroscopy and Acoustic Sensing in Characterization of Blood and Pancreatic Ductal Adenocarcinoma Derived Extracellular Vesicles.- Floating Films of Biological Importance at the Liquid-Gas Interface – Langmuir Monolayer Approach.- Protein Analysis by FT-IR/ATR Spectroscopy.