The Microflow Cytometer

This volume describes the continuing development of inexpensive, portable flow cytometers through incorporation of microfluidic technologies and small optical components. The underlying microfluidic theories essential for microflow cytometry are discussed in detail, as are advances that are representative of the current state-of-the-art. Design and fabrication strategies for these innovative component technologies are subsequently presented by numerous research groups leading the field. Integration of the components into functional prototype devices for analysis and manipulation of particles and cells is reviewed, while multiple currently available commercial systems will be examined to highlight both strengths and areas for improvement.

The field of microfluidic flow cytometry is growing at a fast rate. This volume describes the development of inexpensive, portable flow cytometers through incorporation of microfluidic technologies and small optical components. The book discusses the underlying microfluidic theories essential for microflow cytometry, as well as advances that are representative of the current state-of-the-art. Numerous research groups leading the field are currently presenting design and fabrication strategies for these innovative component technologies, and the book reviews integration of the components into functional prototype devices for analysis and manipulation of particles and cells. It also examines multiple currently available commercial systems to highlight both strengths and areas for improvement.

The History of Flow Cytometry and Sorting, H ShapiroAnalysis of Single Cells Using Lab-on-a-Chip Systems, T PreckelLuminex Personal Flow Cytometers, W RothThe Accuri C6 Flow Cytometer, C Rich & G HowesCapillary Flow Cytometry, D King et al.Sheathless Particle Focusing, S Choi et al.Two-Dimensional Particle Focusing, J Shin & M LadischThree-Dimensional Particle Focusing, P HowellFluidic Control: Pumps and Valves, S Zheng et al.Integrated Optics, Y Hosseini & K KalerPolymer Photonics, D Leuenberger & M RamuzElectrical Detection, M Di BerardinoElectronics, J Erickson et al.Optical Micro-FACS, K Patel & T PerroudRaman-based Label-free Cell Analysis and Sorting, J ChanAutonomous Pathogen Detection System, J Dzenitis & A MakarewiczLaser-based Fabrication with Integrated Optical Waveguides, M Dugan et al.Systems Integration, J Kim et al.

Frances Ligler is a senior scientist at the US Naval Research Laboratory's Center for Bio/Molecular Science and Engineering. She has received numerous medals and awards, including the Women in Science and Engineering Outstanding Achievement in Science Award.