The Laboratory Mouse

With the advent of transgenic and other genetic engineering technologies, the versatility and usefulness of the mouse as a model in biomedical research has soared. Revised to reflect advances since the second edition, The Laboratory Mouse continues to be the most accessible reference on the biology and care of the mouse in research settings. This guide presents basic information and common procedures in detail to provide a quick reference source for investigators, technicians, and caretakerson the humane care and use of the mouse. The new edition adds information on novel technologies such as CRISPR-Cas andon housing systems and management practices; itcovers new concepts such as pain assessment by facial expression and the importance of nest-building as an assessment tool of well-being. There are now expanded sections on anesthesia and analgesia, and on behavior and enrichment. An ideal quick reference for investigators, technicians, and animal caretakers charged with the care and/or use of mice in a research setting, this book will be particularly valuable to those new to working with mice who need to start research programs using these animals.

Preface. About the Authors. Important Biological Features. Introduction. Stocks and Strains. Nomenclature. Anatomic and Physiologic Features. Normative Values. Hematology. Immunodeficient Mice. Wild Mice. Behavior, Well-being, and Enrichment. References. Husbandry. Housing. Barriers and Containment. Personal Protective Equipment (PPE). Special Considerations for Immunodeficient Mice. Environment. Sanitation and Pest Control. Nutrition. Water. Breeding. Identification and Record Keeping. Transportation. References. Management. Regulatory Agencies and Compliance. Institutional Animal Care and Use Committee (IACUC). Occupational Health and Zoonotic Diseases. References. Clinical Medicine. Basic Veterinary Supplies. Physical Examination of the Mouse. Common Spontaneous and Noninfectious Diseases. Common Infectious Diseases. Treatment and Supportive Care of Sick Mice. Clinical Endpoints. Treating Disease on a Colony Basis. References. Preventative Medicine. Receiving. Testing of Biological Materials. Health Surveillance and Monitoring. Disease Prevention through Sanitation. References. Experimental Methodology. Restraint. Sampling Methods. Compound Administration. Anesthesia and Analgesia. Euthanasia. Necropsy. References. Resources and Additional Information. Organizations. Publications. Electronic Resources. Appendix A: Suggested cassette numbering system and some trimming suggestions.

Mark A. Suckow is Associate Vice President for Research, Attending Veterinarian, and Professor of Biomedical Engineering at the University of Kentucky, in Lexington, KY. Dr. Suckow earned the degree of Doctor of Veterinary Medicine from the University of Wisconsin in 1987 and completed a post-doctoral residency program in laboratory animal medicine at the University of Michigan in 1990. He is a Diplomate of the American College of Laboratory Animal Medicine. Dr. Suckow has published over 100 scientific papers and chapters in books. He is a Past President of the American Association for Laboratory Animal Science, Past President of the American Society of Laboratory Animal Practitioners, and serves on the Council on Accreditation of AAALAC, International. Sara Hashway is the Director of the Office of Animal Resources, Attending Veterinarian, and Assistant Research Professor of Psychology and Neuroscience at the University of Colorado, Boulder in Boulder, CO. Dr. Hashway earned her veterinary degree from the University of Georgia in 2010 and completed a post-doctoral fellowship in Laboratory Animal Medicine at the University of Michigan in 2013. She is a Diplomate of the American College of Laboratory Animal Medicine and serves as an ad hoc consultant for AAALAC, International. She has published multiple scientific papers and co-authored the chapter "The Translational Potential of Rats" for the third edition of the ACLAM Series book The Laboratory Rat. Kathleen R. Pritchett-Corning is the Attending Veterinarian and Director, Office of Animal Resources, at the Harvard University Faculty of Arts and Sciences and an Affiliate Assistant Professor in the Department of Comparative Medicine at the University of Washington. Dr. Pritchett-Corning received her B.S. and her D.V.M. from Washington State University and her post-doctoral training from the University of Washington. She became a Diplomate of the American College of Laboratory Animal Medicine in 2002 and has held positions at the University of Washington, the Jackson Laboratory, and Charles River. In 2015, she received the AALAS Pravin Bhatt Scientific Excellence Award. Dr. Pritchett-Corning has worked primarily with mice for 30 years and has authored more than 80 peer-reviewed publications.