The Immune Synapse Methods and Protocols

The Immune Synapse: Past, Present, and Future.- Analyzing Actin Dynamics of the Immunological Synapse.- Analysis of Microtubules and Microtubule-Organizing Centre at the Immune Synapse.- Analyzing the Dynamics of Signaling Microclusters.- Reconstitution of TCR Signaling using Supported Lipid Bilayers.- Plasma Membrane Sheets for Studies of B Cell Antigen Internalization From Immune Synapses.- Studying The Dynamics Of TCR Internalization At The Immune Synapse.- T Cell Receptor Activation of NF-?b in Effector T Cells: Visualizing Signaling Events Within and Beyond the Cytoplasmic Domain of the Immunological Synapse.- Imaging Vesicular Traffic at the Immune Synapse.- Analysis of TCR Recycling at the Immune Synapse.- Simultaneous Membrane Capacitance Measurements and TIRF Microscopy to Study Granule Trafficking at Immune Synapses.- Mathematical Modeling of Synaptic Patterns.- Super-Resolution Analysis of TCR-Dependent Signaling: Single Molecule Localization Microscopy.- Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse.- Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes.- Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains during Immunological Synapse Formation using Donor-Quenching FRET and FLIM-FRET.- Revealing the Role of Microscale Architecture in Immune Synapse Function through Surface Micropatterning.- Spatial Control of Biological Ligands on Surfaces.- Probing Synaptic Biomechanics using Micropillar Arrays.- Microchannels for Study of Immunological Synapses and Kinapses.- Purification of LAT Containing Membranes from Resting and Activated T Lymphocytes.- Quantitative Phosphoproteomic Analysis of T-Cell Receptor Signaling.- Imaging Asymmetric T Cell Division.- Ultrastructure Of Immune Synapses.- Systems Imaging of the Immune Synapse.- Comprehensive Analysis of Immunological Synapse Phenotypes using Supported Lipid Bilayers.- Studying Immunoreceptor Signaling in Human T Cells using Electroporation of In Vitro Transcribed mRNA.- A Protein Expression Tool-Kit for Studying Signalling in T Cells.- Imaging The Effector CD8 Synapse.- The Mast Cell Antibody-Dependent Degranulatory Synapse.- Measurement of Lytic Granule Convergence after Formation of an NK Cell Immunological Synapse.- Studying the T Cell-Astrocyte Immune Synapse.- Aberrant Immunological Synapses Driven by Leukemic Antigen Presenting Cells.- Studying the Immune Synapse in HIV-1 Infection.- In Vivo Imaging of T Cell Immunological Synapses and Kinapses in Lymph Nodes.- Studying Dendritic Cell-T Cell Interactions Under In Vivo Conditions.