T-Follicular Helper Cells Methods and Protocols

The volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical models to study GC dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, T-Follicular Helper Cells: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

Complete Visualization of T Follicular Helper Cells in Germinal Centers.- In vivo imaging of Tfh cells.- Circulating T Follicular Helper Subsets in Human Blood.- Identification of Human Circulating and Tissue T Follicular Regulatory (Tfr) Cells by Flow Cytometry.- Assessment of circulating Tfh and Tfr by mass cytometry.- Identification of T peripheral helper (Tph) cells.- Identification of non-classical follicular T cells.- Identification of follicular T cells in the gut.- Intravital imaging of Tfh dynamics.- A method to analyze models of the dynamics of GCs.- Identification of Antigen Presenting Cell subsets supporting human Tfh differentiation Lamine Alaoui, Melanie Durand, and Elodie Segura.- The use of peptide-MHCII tetramers to identify antigen-specific T follicular helper and T follicular regulatory cells.- Assessment of the TCR repertoire of human circulating T follicular helper cells.- Assessing in vivo T cell-dependent antigen-specific antibody responses.- Tfh-mediated and Tfr-suppressed antigen driven IgG and IgE assays.- Assessment of the impact of cytokines on TFH, TREG, and TFR cell populations after influenza infection.- Evaluation of human circulating T follicular helper cells in influenza- and SARS-CoV-2-specific B cell immunity.- Detection of T follicular helper cells and T follicular regulatory cells in experimental Sjögren’s syndrome.- Identification of Human T Follicular Cells in Ectopic Lymphoid Structures.- Understanding T Follicular helper cells in asthma through murine models of allergic airway disease.- Therapeutic modulation of T follicular helper cells by low-dose IL-2 treatment.- 3D tissue explant and single-cell culture systems for ex vivo drug testing on Tfh cells derived from human adenoid tonsils.