Strigolactones Methods and Protocols

This volume presents the most useful laboratory protocols in strigolactones(SL) research. Chapters guide readers through wet-lab paths, issues around stability, protocols to evaluate SL activity, effects towards soil inhabitants such as parasitic plants, mycorrhizal and non-mycorrhizal fungi, nodulating bacteria, and protocols to assess effects on plant development are discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Strigolactones: Methods and Protocols aims to deliver a clear-cut and standardized set of experimental protocols to a broad scientific community.

Evaluation and Quantification of Natural Strigolactones from Root Exudates.- Isolation and Identification of Naturally Occurring Strigolactones.- Chemical Synthesis of Triazole-Derived Suppressors of Strigolactone Functions.- Synthesis of Simple Strigolactone Mimics.- Synthesis of Strigolctones Analogues and Evaluation of their Stability in Solution.- Strigolactone-like Bioactivity Via Parasitic Plant Germination Bioassay.- Evaluation of the Effect of Strigolactones and Synthetic Analogues On Fungi.- Analysing the Effect of Strigolactones on the Motility Behaviour of Rhizobia.- Chemotropic Assay for Testing Fungal Response to Strigolactones and Strigolactone-Like Compounds.- Methods for Phenotyping Shoot Branching and Testing Strigolactone Bioactivity for Shoot Branching in Arabidopsis and Pea.- Bioassays for the Effects of Strigolactones and Other Small Molecules on Root and Root Hair Development.- Methods for Medium-Scale Study of the Biological Effects of Strigolactone-Like Molecules on the Moss Physcomitrella patens.- Controlled Assays for Phenotyping the Effects of Strigolactone-like Molecules on Arbuscular Mycorrhiza Development.- Application of Strigolactones to Plant Roots to Influence the Formation of Symbioses.- Evaluation of the Bioactivity Oof Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay.- A Protoplast-based Bioassay to Quantify Strigolactone Activity in Arabidopsis using StrigoQuant.- Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies.- The use of Differential Scanning Fluorimetry to Assess Strigolactone Receptor Function.- Structural Analysis of Strigolactone-Related Gene Products.