Stem Cells and Lineage Commitment Methods and Protocols

This detailed volume explores a variety of techniques used to study lineage commitment in stem cells. Further elucidation of the process that stem cells undergo on their way to becoming more specified cell types is vital for a more complete understanding of cell biology and overall physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment.- Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin.- Targeted Gene Silencing by Using GapmeRs in Differentiating Human Induced Pluripotent Stem Cells (hiPSC) Towards Pancreatic Progenitors.- Retinoic Acid-Mediated Differentiation of Mouse Embryonic Stem Cells to Neuronal Cells.- Isolation and Functional Analysis of Myoepithelial Cells from Adult Mouse Submandibular Glands.- Chromosomal Analysis in Lineage-Specific Mouse Hematopoietic Stem Cells and Progenitors.- Easy and Rapid Methods for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Human Umbilical Wharton’s Jelly-Derived Mesenchymal Stem Cells.- Encapsulation of MSCs in PRP-Derived Fibrin Microbeads.- Assessing Neuronogenic-vs-Astrogenic Bias of Neural Stem Cells via In Vitro Clonal Assay.- Stem Cell-Based Modeling Protocol for Parkinson's Disease.-Standard Operating Procedure for Production of Mouse Brown Adipose Tissue-Derived Mesenchymal Stem Cells.- Development and Validation of Type 2 Diabetic Zebrafish Model for Cell-Based Treatments.- Neuromuscular Junction-on-a-Chip for Amyotrophic Lateral Sclerosis Modeling.- Primary Human Leukemia Stem Cell (LSC) Isolation and Characterization.- GMP-Compliant Mesenchymal Stem Cell-Derived Exosomes for Cell Free Therapy in Cancer.- Establishing Brain Tumor Stem Cell Culture from Patient Brain Tumors and Imaging Analysis of Patient-Derived Xenografts.- An Optimized Protocol for PiggyBac-Induced iPSC Generation from hPBMCs by Automatic Electroporation.- Signalling Pathways in Trans-Differentiation of Mesenchymal Stem Cells: Recent Advances.