Stem Cell Nanotechnology Methods and Protocols

Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.  As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

Propagation and Differentiation of Human Wharton’s Jelly Stem Cells on Three Dimensional Nanofibrous Scaffolds.- Composite Electrospun Nanofibers for Influencing Stem Cell Fate.- In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells.- Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells.- Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing.- Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiation of Mesenchymal Stem Cells.- Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer.- Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-Cultures.- Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers.- General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds.- Mesenchymal Stem Cells and Nano-Structured Surfaces.- Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds.- Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Encapsulated Neural Stem Cells.