Somatic Cell Nuclear Transfer Technology

This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.

Cloning by SCNT: integrating technical and biology-driven advances.- Epigenetic reprogramming and somatic cell nuclear transfer.- Early cell specification in mammalian fertilized and somatic cell nuclear transfer embryos.- Mitochondrial inheritance following nuclear transfer: from cloned animals to patients with mitochondrial disease.- Stem cells as nuclear donors for mammalian cloning.- Animal Transgenesis and cloning: combined development and future perspectives.- Mouse cloning using outbred oocyte donors and non-toxic reagents.- Somatic cell nuclear transfer in rabbits.- Production of cloned pigs by handmade cloning.- Somatic Cell Nuclear Transfer in Pigs.- Somatic cell nuclear transfer using freeze-dried protaminized donor nuclei.- Cattle cloning by somatic cell nuclear transfer.- Production of water buffalo SCNT embryos by handmade cloning.- Bovid interspecies somatic cell nuclear transfer with ooplasm transfer.- Horse somatic cell nuclear transfer using zona pellucida intact and zona-free oocytes.- A modified handmade cloning method for dromedary camels.- Derivation of bovine primed embryonic stem cells from somatic cell nuclear transfer embryos.