Skin Stem Cells Methods and Protocols

During the last decade, an increased interest in somatic stem cells has led to a flurry of research on one of the most accessible tissues of the body: skin.  Much effort has focused on such topics as understanding the heterogeneity of stem cell pools within the epidermis and dermis, and their comparative utility in regenerative medicine applications.  In Skin Stem Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study skin stem cells. These include methods and techniques for the isolation, maintenance and characterization of stem cell populations from skin. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Skin Stem Cells: Methods and Protocols seeks to aid scientists in the further understanding of these diverse cell types and the translation of their biological potential to the in vivo setting.

Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin.- Isolation and Cultivation of Human Scalp Interfollicular Epidermal Stem Cells.- Isolation of Hair Follicle Bulge Stem Cells From YFP-Expressing Reporting Mice.- ­­­Analysis of Bulge Stem Cells from the Epidermis using Flow Cytometry.- Lineage Tracing of Hair Follicle Stem Cells in Epidermal Whole Mounts.- Isolation and Characterization of Cutaneous Epithelial Stem Cells.- Identification and Analysis of Epidermal Stem Cells from Primary Mouse Keratinocytes.- Monitoring the Cycling Activity of Cultured Human Keratinocytes using a CFSE-based Dye Tracking Approach.- Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells.- Analysis of Gene Expression in Skin using Laser Capture Microdissection.- Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to Evaluate the Reliability of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction.- Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors.- Using 3D Culture to Investigate the Role of Mechanical Signalling in Keratinocyte Stem Cells.- In vivo Transplantation Assay at Limiting Dilution to Identify the Intrinsic Tissue Reconstitutive Capacity of Keratinocyte Stem Cells and their Progeny.- Melanoblasts as Multipotent Cells in Murine Skin.- Generation of Human Melanocytes from Induced Pluripotent Stem Cells.- lular Populations Isolated from Newborn Mouse Skin Including Mesenchymal Stem Cells.- Isolation, Characterization and Differentiation of Human Multipotent Dermal Stem Cells.- Isolation and Differentiation of Hair Follicle-derived Dermal Precursors.- Isolation of Mesenchymal Stem Cells from Human Dermis.- Skin Derived Mesenchymal Stem Cells: Isolation, Culture and Characterization.- Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures.- Enrichment of Oral Mucosa and Skin Keratinocyte Progenitor/Stem Cells.-Skin StemCells.