Skeletal Muscle Stem Cells Methods and Protocols

Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells.- Extra Eyelid-Derived Muscle Stem Cells.- Isolation, Culture, and Analysis of Zebrafish Myofibres and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis.- The Satellite Cell Colony Forming Cell Assay as a Tool to Measure Self-Renewal and Differentiation Potential.- Co-Cultures of Macrophages with Muscle Stem Cells or with Fibroadipogenic Precursor Cells from Regenerating Skeletal Muscle.- Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Muscle Stem Cells Using a Seahorse Analyzer: Applicability for Aging Studies.- High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes.- State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts.- Analyses of Mesenchymal Progenitors in Skeletal Muscle by Fluorescence-Activated Cell Sorting and Tissue Clearing.- In Vitro Maturation of Human Pluripotent Stem Cell-Derived Myotubes.- Differentiation of Human Fetal Muscle Stem Cells from Induced Pluripotent Stem Cells.- Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells.- Producing Engraftable Skeletal Myogenic Progenitors from Pluripotent Stem Cells via Teratoma Formation.- Techniques for Injury, Cell Transplantation, and Histological Analysis in Skeletal Muscle.- Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading.- Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique.- Skeletal Muscle Regeneration in Zebrafish.- Methods to Monitor Circadian Clock Function in Skeletal Muscle.- Visualizing MyoD Oscillations in Muscle Stem Cells.- In Vivo Modeling of Skeletal Muscle Diseases Using the CRISPR/Cas9 System in Rats.- In Vivo Investigation of Gene Function in Muscle Stem Cells by CRISPR/Cas9 Mediated Genome Editing.- Exons 45-55 Skipping Using Antisense Oligonucleotides in Immortalized Human DMD Muscle Cells.- In Vivo Evaluation of Exon 51 Skipping in hDMD/Dmd-null Mice.- Functional Analysis of MicroRNAs in Skeletal Muscle.- Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring (MRM) Profiling.- Single-Cell Transcriptomic Analysis of Mononuclear Cell Populations in Skeletal Muscle.- Assay for Transposase-Accessible Chromatin Using Sequencing (ATAC-seq) of Freshly Isolated Muscle Stem Cells.- Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells.- Epitranscriptome Mapping of N6-Methyladenosine using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells.- Visualization of RNA Transcripts in Muscle Stem Cells Using Single-Molecule Fluorescence In Situ Hybridization.- Tissue Clearing and Confocal Microscopic Imaging for Skeletal Muscle.- Three-Dimensional Imaging Analysis for Skeletal Muscle.