1. Single-Molecule Analysis by Biological Nanopores Yuqin Wang and Shuo Huang 2. Optical Tweezers for Manipulation of Single Molecules Guangtao Song and Yan Zeng 3. Single-Molecule Biosensing by Fluorescence Resonance Energy Transfer Ying Lu, Jianbing Ma, and Ming Li 4. DNA Origami as Single-Molecule Biosensors Travis A. Meyer, Qinyi Lu, Kristin Weiss, and Yonggang Ke 5. Single-Molecule Manipulation by Magnetic Tweezers Zilong Guo and Hu Chen 6. Long-Time Recording of Single-Molecule Dynamics in Solution by Anti-Brownian Trapping Quan Wang, Elif Karasu, and Hugh Wilson