RNA Structure and Dynamics

Co-transcriptional Assembly and Native Purification of Large RNA-RNA Complexes for Structural Analyses.- Probing RNA Structures and Interactions using Fluorescence Lifetime Analyses.- Characterizing Fluorescence Properties of Turn-on RNA Aptamers.- Probing Transient Riboswitch Structures via Single Molecule Accessibility Analysis.- Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch using an Interdependent-Sites Binding Model.- Application of Molecular Dynamics to Expand Docking Program’s Exploratory Capabilities and to Evaluate Its Predictions.- Exploring the Binding Process of Cognate Ligand to add Adenine Riboswitch Aptamer by using Explicit Solvent Molecular Dynamics (MD) Simulation.- Prediction of Dynamic RBP-RNA Interactions using PrismNet.- High-resolution Atomic Force Microscopy Imaging of RNA molecules in solution.- 3D Structural Analysis of Long Non-coding RNA by Small Angle X-ray Scattering and Computational Modeling.- Combining Biophysical Methods for Structure-function Analyses of RNA in Solution.- Near-Atomic Resolution Cryo-EM Image Reconstruction of RNA.- Auto-DRRAFTER: Automated RNA Modeling Based on Cryo-EM Density.- X-ray Crystallography to Study Conformational Changes in a TPP Riboswitch.- Preparation of RNA Microcrystals for Serial Femtosecond Crystallography Experiments.- Mix-and-Inject Serial Femtosecond Crystallography to Capture RNA Riboswitch Intermediates.