RNA Editing Methods and Protocols

This volume provides an overview about main RNA editing mechanisms, focusing on their functions in physiological as well as pathological conditions. Chapters guide readers through state- of-the art methodologies to investigate RNA editing through wet and dry approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA Editing: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Substitutional RNA Editing in Plant Organelles

Mizuho Ichinos and Mamoru Sugita

2. Computational Detection of Plant RNA Editing Events

Alejandro A. Edera and M. Virginia Sanchez-Puerta

Huiquan Liu and Jin-Rong Xu

4. C-to-U RNA Editing: From Computational Detection to Experimental Validation

Taga Lerner, Mitchell Kluesner, Rafail Nikolaos Tasakis, Branden S. Moriarity, F.N. Papavasiliou, and  Riccardo Pecori

5. Live Cell Quantification of APOBEC1-mediated RNA Editing: A Comparison of RNA Editing Assays

Martina Chieca, Serena Torrini, and  Silvestro G. Conticello

6. Adenosine to Inosine RNA Editing Enzyme ADAR and microRNAs

Kang Yuting, Dan Ding, and Hisashi Iizasa

Turnee N. Malik, Jean-Philippe Cartailler, and Ronald B. Emeson

8. Discovering A-to-I RNA Editing Through Chemical Methods

Masayuki Sakurai, Shunpei Okada, Hiroki Ueda, and Yang Yuxi

9. ALU A-to-I RNA Editing - Millions of Sites and Many Open Questions

Amos Schaffer and Erez Y. Levano

10. RNA Editing in Human and Mouse Tissues

Harini Srinivasan, Eng Piew Louis Kok, and Meng How Tan

11. Bioinformatics Resources for RNA Editing

Maria Angela Diroma, Loredana Ciaccia, Graziano Pesole, and Ernesto Picardi

Claudio Lo Giudice, Graziano Pesole, and Ernesto Picardi

13. Detection of A-to-I Hyper-Edited RNA Sequences

Roni Cohen-Fultheim and Erez Y. Levanon

Eli Eisenberg

15. MicroRNA Editing Detection and Function: A Combined in silico and Experimental Approach for the Identification and Validation of Putative Oncogenic Targets

Valentina Tassinari, Valeriana Cesarini, Domenico Alessandro Silvestris, Andrea Scafidi, Lorenzo Cucina, and Angela Gallo

16. RNA Editing in Interferonopathies

Loredana Frassinelli, Silvia Galardi, Silvia Anna Ciafrè, and Alessandro Michienzi

17. The Role of RNA Editing in the Immune Response

Sadeem Ahmad, Xin Mu, and Sun Hur

Pedro Henrique Costa Cruz and Yukio Kawahara

Tobias Merkle and Thorsten Stafforst