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Reporter Genes A Practical Guide




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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana Press

Edizione: 2007





Trama

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.





Sommario

Methodologies for Staining and Visualisation of ?-Galactosidase in Mouse Embryos and Tissues.- Immunohistochemical Detection of ?-Galactosidase or Green Fluorescent Protein on Tissue Sections.- Detection of Reporter Gene Expression in Murine Airways.- Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques.- Fluorescent Proteins for Cell Biology.- Detection of GFP During Nervous System Development in Drosophila melanogaster.- Autofluorescent Proteins for Flow Cytometry.- Fluorescent Protein Reporter Systems for Single-Cell Measurements.- Subcellular Imaging of Cancer Cells in Live Mice.- Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects.- Green Fluorescent Protein as a Tracer in Chimeric Tissues.










Altre Informazioni

ISBN:

9781588297396

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 235 x 155 mm
Formato: Copertina rigida
Illustration Notes:X, 158 p.
Pagine Arabe: 158
Pagine Romane: x


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