Rat Genomics Methods and Protocols

Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems. The volume extensively covers topics including genome sequencing, quantitative trait loci mapping, and the identification of single nucleotide polymorphisms as well as the development of transgenic technologies such as nuclear cloning, lentiviral-mediated transgenesis, gene knock-down using RNA interference, gene knock-out by mutagenesis, and zinc finger nucleases plus exciting advances in the obtention of rat embryonic cell lines. As a volume in the highly successful Methods in Molecular Biology™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Comprehensive and up-to-date, Rat Genomics: Methods and Protocols thoroughly covers the current techniques used in labs around the world and overviews the applications of the data obtained, making it certain to be useful to the scientific community as a key source of references and methods.

1. The Rat: A Model Used In Biomedical Research Howard J. Jacob 2. Genetic Mapping and Positional Cloning Timothy J. Aitman, Enrico Petretto, and Jacques Behmoaras 3. Sequencing of the Rat Genome and Databases Elizabeth A. Worthey, Alexander J. Stoddard, and Howard J. Jacob 4. Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators) Louis-Marie Houdebine 5. Inducible and Conditional Promoter Systems to Generate Transgenic Animals Yoji Hakamata and Eiji Kobayashi 6. Generation of Transgenic Rats by Microinjection of Short DNA Fragments Séverine Ménoret, Séverine Remy, Claire Usal, Laurent Tesson, and Ignacio Anegon 7. Generation of Transgenic Rats Using YAC and BAC DNA Constructs Ri-ichi Takahashi and Masatsugu Ueda 8. The Use of Lentiviral Vectors to Obtain Transgenic Rats Séverine Remy, Tuan Huy NGuyen, Séverine Ménoret, Laurent Tesson, Claire Usal, and Ignacio Anegon 9. Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA Masumi Hirabayashi and Shinichi Hochi 10. Procedures for Somatic Cell Nuclear Transfer in the Rat Jean Cozzi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Yacine Cherifi, and Qi Zhou 11. ENU Mutagenesis to Generate Genetically Modified Rat Models Ruben van Boxtel, Michael N. Gould, Edwin Cuppen, and Bart M. G. Smits 12. Establishment of Embryonic Stem Cells from Rat Blastocysts Masaki Kawamata and Takahiro Ochiya 13. Derivation, Culture, and in vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts Simon-Pierre Demers and Lawrence C. Smith 14. Interference RNA for in vivo Knock-Down of Gene Expression orGenome-Wide Screening Using shRNA Silvère Petit and Kader Thiam 15. Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases Aron M. Geurts, Gregory J. Cost, Séverine Rémy, Xiaoxia Cui, Laurent Tesson, Claire Usal, Séverine Ménoret, Howard J. Jacob, Ignacio Anegon, and Roland Buelow 16. Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics Nan Mei, James C. Fuscoe, Edward K. Lobenhofer, and Lei Guo 17. Generation of Congenic and Consomic Rat Strains Dominique Lagrange and Gilbert J. Fournié 18. Generation of Rat 'Supersonic' Congenic/Conplastic Strains Using Superovulation and Embryo Transfer Vladimír Landa, Václav Zídek, and Michal Pravenec 19. Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms Laurent Tesson, Séverine Rémy, Séverine Ménoret, Claire Usal, and Ignacio Anegon 20. A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites Elizabeth C. Bryda and Beth A. Bauer 21. Cryopreservation and Orthotopic Transplantation of Rat Ovaries Martina Dorsch and Dirk Wedekind 22. Techniques for In Vitro and In Vivo Fertilization in the Rat Naomi Kashiwazaki, Yasunari Seita, Akiko Takizawa, Naoki Maedomari, Junya Ito, and Tadao Serikawa 23. Rat Strain Repositories Birger Voigt 24. Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases Yvonne K. Urbach, Felix Bode, Huu Phuc Nguyen, Olaf Riess, and Stephan von Hörsten 25. Rat Genomics Applied to Psychiatric Research Marie-Pierre Moisan and André Ramos 26. Genomics Studies of Immune-Mediated Diseases Using the BN-LEW Rat Model Isabelle Bernard, Gilbert Fournié, and Abdelhadi