Quantitative Real-Time PCR Methods and Protocols

A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers.- Parameters for Successful PCR Primer Design.- MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing.- Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation.- A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR.- Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR).- Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR).- Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine.- A General Protocol for Accurate Gene Expression Analysis in Plants.- Gene Expression Analysis in Bacteria by RT-qPCR.- Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR.- Molecular Monitoring of Chronic Myeloid Leukemia.-Normalization in Human Gliomas Tissue.- qPCR Applications for the Determination of the Biological Age.- QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis.- Digital PCR and the QuantStudio™ 3D Digital PCR System.