Proteomics From Protein Sequence to Function

Following the announcement of the draft sequence of the human genome and the completion of many others, attention is now increasingly turning to the analysis of the proteins encoded by genomes - proteomics. This is a rapidly emerging research area and this book reviews recent developments in proteomics and compares them with current genomics technologies. The book is written by experts, from academia and industry, who review their area in a concise yet easy to understand style, providing a comprehensive review volume that integrates genomics with proteomics. This is the first book to address the subject at an undergraduate level.

Bridging genomics and proteomics. Methods for measurement of gene (mrna) expression. Two-dimensional polyacrylamide gel electrophoresis for proteome analysis. Detecting proteins in polyacrylamide gels and on electroblot membranes. Mass spectrometry-based methods for protein identification and phosphorylation site analysis. Image analysis of 2 dimensional gels. Enhancing high-throughput proteome analysis: the impact of stable isotope labeling. The automation of proteomics: technical and informatic solutions for high-throughout protein analysis. Message in a bottle neck: a complete approach to automating proteome analysis. Novel approaches to protein expression analysis. Application of proteome analysis to drug development and toxicology. Phage antibodies as tools for proteomics. Glycobiology and proteomics. Proteomics in an academic environment. Proteomics as a tool for plant genetics and breeding.