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karamanos nikos k. (curatore) - proteoglycans

Proteoglycans Methods and Protocols

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Lingua: Inglese


Pubblicazione: 01/2023
Edizione: 1st ed. 2023


This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.



Part I: Glycosaminoglycans: Strategies for Detection and Structure Characterization
1. Detection of Glycosaminoglycans in Biological Specimens 
Shaukat A Kha, FNU Nidhi, Paige C. Amendu, and Shunji Tomatsu

2. Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization Of Chondroitin Sulfate Proteoglycans
Tadahisa Mikami and Hiroshi Kitagawa

3. Proteoglycans: A Tool for detecting hyaluronan by ELISA-like Methods
Daiana L. Vitale, Rosalía I. Cordo-Russo, and Laura Alaniz

4. Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix
Arianna Parnigoni, Paola Moretto, Simona Rovera, Manuela Viola, Evgenia Karousou, Alberto Passi, and Davide Vigetti

5. Urinary Glycosaminoglycans: Characterization and Quantification
Naima Fdil and Es-Said Sabir

6. HPLC-MS characterization of tissue-derived heparan sulfate and chondroitin sulfate
Gábor Tóth, Lilla Turiák

7. Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues 
Chrysostomi Gialeli and Isabel Gonçalves

8. Studying the effects of Glycosaminoglycans in Cell Morphological Aspect with Scanning Electron Microscopy
Christos Koutsakis, Marco Franchi, Anastasia-Gerasimoula Tavianatou, Valentina Masola, and Nikos K. Karamanos

Part II: Proteoglycans: Separation, Analysis, and Interactions
9. Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity
Jinyang Zeng-Brouwers, Lisa Sophie Huber, Rosetta Merline, Jonel Trebicka, Malgorzata Wygrecka, and Liliana Schaefer

10. Analysis of Extracellular Vesicle-associated Proteoglycans
Juliana Poças and Mattias Belting

11. Analysis of Aggrecan Glycanation by Western Blot in Cell Culture
Chiara Gramegna Tota, Alessandra Leone, Antonio Rossi, and Chiara Paganini

12. Repulsive Scaling Replica Exchange Molecular Dynamics in Modeling Protein-Glycosaminoglycan Complexes
Mateusz Marcisz, Martyna Maszota-Zieleniak, and Sergey A. Samsonov

13. In vitro investigation of hyaluronan/CD44 network
Theodore Karalis, Paraskevi Heldin, and Spyridon S. Skandalis

14. Separation and Identification of Native Proteoglycans by Composite 
Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting
James Melrose

15. Building, Visualizing, and Analyzing Glycosaminoglycan-Protein Interaction Networks
Sylvie Ricard-Blum

Part III: Proteoglycans related Molecules (Enzymes, Inhibitors, and Epigenetics)
16. An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying Kinetics
Edward Hammond and Vito Ferro

17. A method for Urinary Trypsin Inhibitor (UTI) purification combining anion-exchange chromatography enrichment and preparative SDS-PAGE
Gabriele Nieddu, Marilena Formato, and Antonio Junior Lepedda

18. Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase
Qingdong Zhang, Danrong Lu, and Fuchuan Li

19. Evaluating the Effects of microRNAs on Proteoglycans and Matrix Constituents’ Expression and Functional Properties
Zoi Piperigkou and Nikos K. Karamanos 

20. Proteoglycan Expression Studied by microRNAs
Nancy Adriana Espinoza-Sanchez, Fabian Troschel, Burkhard Greve, and Martin Götte

21. Modeling and simulations of Extra Cellular Glycoproteins
Rajas M Rao, Nicolas Belloy, Jean-Marc Crowet, Manuel Dauchez, and Stéphanie Baud

Altre Informazioni



Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 823 gr
Formato: Copertina rigida
Illustration Notes:XIV, 317 p. 67 illus., 59 illus. in color.
Pagine Arabe: 317
Pagine Romane: xiv

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