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karamanos nikos k. (curatore) - proteoglycans

Proteoglycans Methods and Protocols

Name: Proteoglycans
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Author: Karamanos Nikos K. (Curatore)
ISBN: 9781071629451

karamanos nikos k. (curatore)

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Genere:Libro

Lingua: Inglese

Editore:

Humana

Pubblicazione: 01/2023

Edizione: 1st ed. 2023

Trama

This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.

Sommario

Part I: Glycosaminoglycans: Strategies for Detection and Structure Characterization

1. Detection of Glycosaminoglycans in Biological Specimens

Shaukat A Kha, FNU Nidhi, Paige C. Amendu, and Shunji Tomatsu

2. Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization Of Chondroitin Sulfate Proteoglycans

Tadahisa Mikami and Hiroshi Kitagawa

3. Proteoglycans: A Tool for detecting hyaluronan by ELISA-like Methods

Daiana L. Vitale, Rosalía I. Cordo-Russo, and Laura Alaniz

4. Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix

Arianna Parnigoni, Paola Moretto, Simona Rovera, Manuela Viola, Evgenia Karousou, Alberto Passi, and Davide Vigetti

5. Urinary Glycosaminoglycans: Characterization and Quantification

Naima Fdil and Es-Said Sabir

6. HPLC-MS characterization of tissue-derived heparan sulfate and chondroitin sulfate

Gábor Tóth, Lilla Turiák

7. Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues

Chrysostomi Gialeli and Isabel Gonçalves

8. Studying the effects of Glycosaminoglycans in Cell Morphological Aspect with Scanning Electron Microscopy

Christos Koutsakis, Marco Franchi, Anastasia-Gerasimoula Tavianatou, Valentina Masola, and Nikos K. Karamanos

Part II: Proteoglycans: Separation, Analysis, and Interactions

9. Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity

Jinyang Zeng-Brouwers, Lisa Sophie Huber, Rosetta Merline, Jonel Trebicka, Malgorzata Wygrecka, and Liliana Schaefer

10. Analysis of Extracellular Vesicle-associated Proteoglycans

Juliana Poças and Mattias Belting

11. Analysis of Aggrecan Glycanation by Western Blot in Cell Culture

Chiara Gramegna Tota, Alessandra Leone, Antonio Rossi, and Chiara Paganini

12. Repulsive Scaling Replica Exchange Molecular Dynamics in Modeling Protein-Glycosaminoglycan Complexes

Mateusz Marcisz, Martyna Maszota-Zieleniak, and Sergey A. Samsonov

13. In vitro investigation of hyaluronan/CD44 network

Theodore Karalis, Paraskevi Heldin, and Spyridon S. Skandalis

14. Separation and Identification of Native Proteoglycans by Composite

Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting

James Melrose

15. Building, Visualizing, and Analyzing Glycosaminoglycan-Protein Interaction Networks

Sylvie Ricard-Blum

Part III: Proteoglycans related Molecules (Enzymes, Inhibitors, and Epigenetics)

16. An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying Kinetics

Edward Hammond and Vito Ferro

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Proteoglycans Methods and Protocols

karamanos nikos k. (curatore)

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Humana

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