Protein Arrays Methods and Protocols

This volume discusses the latest methods and applications of protein microarrays and helps researchers harness its power in their work. The chapters in this book explore protein microarray’s development and fabrication, strategies for their data analysis, and wide-reaching applications in biology and disease research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Arrays: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, laboratory scientists, and industry professionals who wish to learn more about protein microarray technology.

Chapter 3 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

 Printing High-Density Human Protein Fragments on Epoxy-Treated Slides.- Building Reversed-Phase Microarrays on Low-Cost Biosensing Chips: Application to Multiplexed Detection of Food Allergies.- Bead-Based Suspension Array Using Peptides as Antigens to Capture and Identify Serum Antibodies.-  On-Array Citrullination of Protein Microarrays.- High-Throughput Post-Translational Modification Analyses by Reverse Phase Protein Array.- Optimization of Serum and Microarray Concentrations for a Semiquantitative Multiplex Indirect Immunoassay.- Protein Microarray Analysis with GenePix.- Evaluating Cross-Reactivity in Custom-Made Multiplex Bead-Based Antibody Microarrays: A Methodological Approach.- SomaScan Bioinformatics: Normalization, Quality Control, and Assessment of Pre-Analytical Variation.- Proteomics by qPCR Using Proximity Extension Assay (PEA).- Multi-Array Technology for Secreted Cytokine Profiling in a Nano-Aerosol Exposed 3D Human Alveolar Cell Model.- Use of Humanized NFAT-Dsred RBL Reporter for Detection of Allergen-Specific IgE Sensitization in Human Serum in 96-Well Microarray Format.- A Competitive Ligand-Binding Assay for Simultaneous Detection of Neutralizing Antibodies to Both Arms of a Bispecific Drug.- Alternative Matrices for Protein Biomarker Analysis by qPCR using Proximity Extension Assay (PEA).- Cytokine Antibody Microarray-Based Proteomic Strategies for Characterizing the Dysregulation of Cytokines Disease-Specific.- Unravelling Immunogenic Cell Death Mechanisms by Functional Proteomics Arrays.- Combination of Antibody Arrays to Characterize the Effect of Neuropathological Stimulus in Human Olfactory Neuroepithelial Cells.