Pre-Clinical Models Techniques and Protocols

A Guide to Mass Spectrometry-based Quantitative Proteomics.- Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors.- Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes.- The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents.- The Forced Swim Test for Depression-like Behaviour in Rodents.- Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice.- The Nest Building Test in Mice for Assessment of General Well-being.- Mouse Free Dyadic Social Interaction Test.- The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour.- The Y-maze for Assessment of Spatial Working and Reference Memory in Mice.- Maturation of a Human Oligodendrocyte Cell Line.- Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan.- Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur.- Characterization of an Animal Model of Autism and Social Interaction.- Assaying Reproductive Capacity in Female Rodents.- Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents.- An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry.- Characterization of the db/db Mouse Model of Type 2 Diabetes.- Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes.- Characterization of Human Transplantable Insulinoma Cells.- Preparation of Islets from Rat Pancreas and Assessment of Islet Function.- Cryopreservation of Red Blood Cells.- Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation.- Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors.- The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis.- Generation of Human Pyruvate Carboxylase Knockout Cell Lines UsingRetrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research.- The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity.- OVA-induced Allergic Airway Inflammation Mouse Model.- Description of Ovariectomy Protocol in Mice.- A Rat Eye Lens Model of Cataract Formation.- A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue.- Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line– a Preclinical Model for Type 2 Diabetes.