Poly(ADP-Ribose) Polymerase Methods and Protocols

Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High Performance Liquid Chromatography, and Capillary Electrophoresis.- Detecting and Quantifying pADPr In Vivo.- Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage.- Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly(ADP-Ribose).- Detecting Poly(ADP-Ribose) In Vitro and in Cells Using PAR-Trackers.- An Enzyme-Linked Immunosorbent Assay to Quantify Poly(ADP-Ribose) Level In Vivo.- Subcellular Quantitation of ADP-Ribosylation by High Content Microscopy.- A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery.- Immunoprecipitation Using Mono-ADP-Ribosylation Specific Antibodies.- A Clickable NAD⁺ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins.- Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells.- Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer.- Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2), a Cation Channel Activated by ADP-Ribose and Involved in Cell Death.- Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function.- Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrometry.- Cytological Approaches to Visualize Intracellular Dynamics of RNA Binding Proteins at Active Genes in Drosophila.- Chromatin Immunoprecipitation Approach to Determine How PARP1 Domains Affect Binding Pattern to Chromatin.- Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates.- Examining the Effect of PARP-1 Inhibitors on Transcriptional Activity of Androgen Receptor in Prostate Cancer Cells.- Using Drosophila Genetics to Identify Factors that Affect PARP1 Activity In Vivo.- Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System.- TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice.-Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells.- Quantification of PARP7 Protein Levels and PARP7 Inhibitor Target Engagement in Cells Using a Split Nanoluciferase System.- Purification of Recombinant Human PARG and Activity Assays.- Purification of Recombinant Human PARP-3.