Plant Organ Abscission Methods and Protocols

This volume details methods on abscission that can be applied by scientists studying organ abscission. Chapters guide readers through organ abscission on diverse species including rice, Setaria viridis, Arabidopsis, yellow lupine, tomato and Nicotiana benthamiana, rose, oil palm, apple, litchi, Areca catechu, and fir.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Organ Abscission: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Immunocytochemical Detection of Phytohormones and Phytohormone-Related Compounds in Abscission Zones.- Immunolocalization of Auxin in the Rose Petal Abscission Zone.- The Quantification of Jasmonates in Lipid-Rich Tissues by UHPLC-ESI-QQQ-MS/MS in Multiple Reaction Monitoring (MRM).- Analysis of Pectins, Extensins, and Hemicelluloses in Abscission Zones.- Detection of Pectic Polysaccharides in Abscission Zones by Immunolocalization.- Histological Staining and Hydrogen Peroxide Visualization of the Abscission Zone in Setaria viridis.- Detection of Reactive Oxygen Species (ROS) Levels in the Fruitlet Abscission Zone of Litchi.- Physiological and Biochemical Methods used in the Study of Fruitlet Abscission in Areca catechu.- Staining Methods for Visualization of Cellular Damage During Petal Abscission.- Histochemical Assays for Analyzing Abscission Layer Development and Function in Rice.- Polar Lipid Extraction and Analysis During Conifer Needle Abscission.- From the Field to the Lab: Establishing Different Abscission Potentials in Apple Fruitlets for Transcriptomic and Functional Studies.- Virus-Induced Gene Silencing to Unravel the Function of Nicotiana benthamiana Genes Linked to Corolla Abscission.- The Application of Virus-Induced Gene Silencing in the Study of Rose Petal Abscission.- A Methodological Approach to Facilitate Abscission Zone Isolation and Gene Regulation Studies of Rose.- Quantitative Analysis of Tomato Flower Pedicel Abscission and Single-Cell Transcriptome Analysis.