Osteoporosis and Osteoarthritis

This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration. The first half of the book examines cell and molecular biological aspects of osteoarthritis and osteoporosis, while the remainder of the collection focuses on in vivo models of skeletal tissue injury, degeneration, and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Part I: Cellular and Molecular Biology of Osteoarthritis and Osteoporosis

 

1. Isolation of Murine and Human Osteocyte

            Matthew Prideaux, Amber Rath Stern, and Lynda F. Bonewald

 

2. Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

            Roberto Narcisi, Wendy J.L.M. Koevoet, and Gergo J.V.M. van Osch

 

3. A Novel Enzymatic Digestion Approach for Isolation and Culture of Rodent Bone Marrow Mesenchymal Progenitors

            Leilei Zhong, Lutian Yao, and Ling Qin

 

4. Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc

            Guus G.H. van den Akker, Andy Cremers, Donatus A.M. Surtel, Willem Voncken, and Tim J.M. Welting

 

5. Engineering Cartilage Tissue by Co-Culturing of Chondrocytes and Mesenchymal Stromal Cells

            Yao Fu, Carlo A. Paggi, Amel Dudakovic, Andre J. van Wijnen, Janine Post, and Marcel Karperien

 

6. Generation of Induced Pluripotent Stem Cells

            David R. Deyle

 

7. Specimen Preparation for Single Cell Sequencing Analysis of Skeletal Cells

            Shawon Debnath and Matthew Greenblatt

 

8. Mapping 5-Hydroxymethylcytosine (5hmC) Modifications in Skeletal Tissues Using High-Throughput Sequencing

            Fiorella Carla Grandi and Nidhi Bhutani

 

9. Using FRAP to Quantify Changes in Transcription Factor Dynamics after Cell Stimulation: Cell Culture, FRAP, Data Analysis, and Visualization

            Kannan Govindaraj and Janine N. Post

 

10. Quantitative Molecular Models for Biological Processes: Modeling of Signal Transduction Networks with ANIMO

            Sakshi Khurana, Janet Huisman, Stefano Schivo, and Janine N. Post

 

Part II: In Vivo Models of Skeletal Tissue Injury, Degeneration, and Repair

 

11. Generation and Characterization of Mouse Models for Skeletal Disease

            Gabrielle E. Foxa, Ye Liu, Lisa M. Turner, Alexander G. Robling, Tao Yang, and Bart O. Williams

 

12. Drill Hole Models to Investigate Bone Repair

            Zhijun Li and Jill Helms

 

13. Generation and Experimental Outcomes of Closed Femoral Fracture in Mice

            Joseph L. Roberts, Christopher W. Kinter, and Hicham Drissi

 

14. Mouse Models of Osteoarthritis: Surgical Model of Post-Traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus

            Kirsty L. Culley, Purva Singh, Samantha Lessard, Mengying Wang, Brennan Rourke, Mary B. Goldring, and Miguel Otero

 

15. Immunostaining of Skeletal Tissues

            Crystal Idleburg, Madelyn R. Lorenz, Elizabeth N. DeLassus, Erica L. Scheller, and Deborah J. Veis

 

16. Mapping Regional Cortical Bone Responses to Local Changes in Loading and Systemic Stimuli

            Sara Windahl, Peter J. Delisser, and Gabriel L. Galea

 

17. Pain and Activity Measurements

            David H.H. Molstad and Elizabeth W. Bradley