Organ Regeneration 3D Stem Cell Culture & Manipulation

This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.

Generation of Various Telencephalic Regions from Human Embryonic Stem Cells in Three-Dimensional Culture.- Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture.- 3D Culture for Self-Formation of the Cerebellum from Human Pluripotent Stem Cells through Induction of the Isthmic Organizer.- Reconstitution of a Patterned Neural Tube from Single Mouse Embryonic Stem Cells.- Functional Pituitary Tissue Formation.- Directed Differentiation of Mouse Embryonic Stem Cells into Inner Ear Sensory Epithelia in 3D Culture.- Generation of Functional Thyroid Tissue using 3D-Based Culture of Embryonic Stem Cells.- Functional Tooth Regeneration.- Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells.- Functional Salivary Gland Regeneration.- Generation of a Bioengineered Lacrimal Gland by using the Organ Germ Method.- Generation of Gastrointestinal Organoids from Human Pluripotent Stem Cells.- Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells.- Making a Kidney Organoid using the Directed Differentiation of Human Pluripotent Stem Cells.- Liver Regeneration using Cultured Liver Bud.- Formation of Stomach Tissue by Organoid Culture using Mouse Embryonic Stem Cells.- In Vivo Model of Small Intestine.