Oncogene-Induced Senescence Methods and Protocols

This volume explores a collection of the most recent experimental approaches for the detection, modelling, characterization, and in-depth computational analysis of the biological phenomenon known as Oncogene-Induced Senescence (OIS). The chapters in this book cover topics such as protocols to study OIS in two-dimensional, three-dimensional, and in vivo (animal and human) settings; analyzing OIS using high-throughput bioinformatics; and discriminate OIS from aging. Published in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting to avoid known pitfalls.

Comprehensive and authoritative, Oncogene-Induced Senescence: Methods and Protocols is a valuable resource for basic researchers and clinicians in the oncology field, and a great guide for computational scientists.

In Situ and In Silico Methods for Senescence Identification in Human Liver Diseases.- Current Methodologies to Assess Cellular Senescence in Cancer.- Selective Sorting of Senescent Cell Subpopulations Compatible with Downstream Genomics Pipelines.- Detection and Characterization of Senescent Cells with Flow Cytometry.-    Detecting Senescence in T Cells by Flow Cytometry Using the SA-β-galactosidase Assay.- The DNA Damage Response as an Auxiliary Indicator of Senescence in Cancer: A User-Friendly Toolkit of Markers and Detection Methods.- Precise Mapping of Physiological DSBs Using In-Suspension Break Labelling In Situ and Sequencing (sBLISS).- Detecting Telomeric DNA Damage by Immuno-Telo FISH.- Combined In Situ Transcriptomic and Immunofluorescence to Assess the Effects on DNA Damage and Innate Immune Responses Induced by Bacterial Genotoxin in In Vivo Models.- Two-Replication Round Telomere Strand-Specific Chromosome Orientation Fluorescent In Situ Hybridization.- Identification of Oncogene-Induced Senescence-Associated MicroRNAs.- Global Proteomic Profiling to Unravel Lipofuscin’s Protein Repertoire.- Electron Microscopy to Visualize and Quantify Mitochondrial Structure and Organellar Interactions in Cultured Cells during Senescence.- Isolation and Characterization of EVs Containing Mitochondria from OIS Cells.- Isolation and Characterization of Endothelial Colony Forming Cells (ECFC): Studying Endothelial Senescence for Translational Studies and for Personalized Medicine.-  In Vitro Modeling of Vascular Senescence.- Studying Cellular Senescence Using the Model-Organism Drosophila melanogaster.- C. elegans as a Screening Platform for Anti-Aging Compounds.- Natural Product-Derived Senotherapeutics: Extraction and Biological Evaluation Techniques.