Neural Reprogramming Methods and Protocols

This detailed book brings together a number of state-of-the-art protocols to generate different types of neural cells through the use of reprogramming technologies. Additionally, the volume explores different aspects of functional evaluation and applications of reprogrammed neural cells as well as in silico methods to aid reprogramming efforts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Isolation and Neuronal Reprogramming of Mouse Embryonic Fibroblasts

            Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall

 

2. Direct In Vitro Reprogramming of Astrocytes into Induced Neurons

            Nesrin Sharif, Filippo Calzolari, and Benedikt Berninger

 

3. Direct Cell Reprogramming of Mouse Fibroblast into Functional Astrocytes Using Lentiviral Overexpression of the Transcription Factors NFIA, NFIB, and SOX9

            Boning Qiu, Ruben J. de Vries, and Massimiliano Caiazzo

 

4. Direct Reprogramming of Fibroblasts to Astrocytes Using Small Molecules

            E Tian, Mingzi Zhang, and Yanhong Shi

 

5. Bcl-2-Assisted Reprogramming of Mouse Astrocytes and Human Fibroblasts into Induced Neurons

            Amel Falco, Rocío Bartolomé-Cabrero, and Sergio Gascón

 

6. Direct Conversion of Human Fibroblasts to Induced Neurons

            Lucia Zhou-Yang, Sophie Eichhorner, Lukas Karbacher, Lena Böhnke, Larissa Traxler, and Jerome Mertens

 

7. Generation of Induced Dopaminergic Neurons from Human Fetal Fibroblasts

            Emilie M. Legault and Janelle Drouin-Ouellet

 

8. Direct Differentiation of Functional Neurons from Human Pluripotent Stem Cells (hPSCs)

            Ruiqi Hu, Xiaoting Zhu, and Nan Yang

 

9. Generation of Motor Neurons from Human ESCs/iPSCs Using Sendai Virus Vectors

            Keiko Imamura, Jitsutaro Kawaguchi, Tsugumine Shu, and Haruhisa Inoue

 

10. Transcription Factor Programming of Human Pluripotent Stem Cells to Functionally Mature Astrocytes for Monocultures and Cocultures with Neurons

            Ella Quist, Henrik Ahlenius, and Isaac Canals

 

11. Single Transcription Factor-Based Differentiation Allowing Fast and Efficient Oligodendrocyte Generation via SOX10 Overexpression

            Katrien Neyrinck and Juan A. García-León

 

12. Transcriptional Profiling During Neural Conversion

            Yohannes Afeworki, Hannah Wollenzien, and Michael S. Kareta

 

13. Functional Assessment of Direct Reprogrammed Neurons In Vitro and In Vivo

            Srisaiyini Kidnapillai and Daniella Rylander Ottosson

 

14. Neurotransmitter Release of Reprogrammed Cells Using Electrochemical Detection Methods

            Andreas Heuer

 

15. Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions

            Juan M. Adrian-Segarra, Bettina Weigel, and Moritz Mall

 

16. CRISPR/Cas9-Genome Engineering in Human Pluripotent Stem Cells for Modeling of Neurological Disorders

            Isaac Canals and Henrik Ahlenius

 

17. Derivation of Adult Human Cortical Organotypic Slice Cultures for Co-Culture with Reprogrammed Neuronal Cells

            Giedre Kvist