Part I: Stroke Models and Surgical Interventions
1. Rodent Stroke Models to Study Functional Recovery and Neural Repair
Daimen R.S. Britsch, Nausheen Syeara, Ann M. Stowe, and Vardan T. Karamyan
2. Subcortical White Matter Stroke in the Mouse: Inducing Injury and Tracking Cellular Proliferation
Miguel Alejandro Marin, Amy J. Gleichman, Andrew J. Brumm, and S. Thomas Carmichael
3. A Low Budget Photothrombotic Rodent Stroke Model
Faisal F. Alamri, Serob T. Karamyan, and Vardan T. Karamyan
4. Photothrombotic Model to Create an Infarct in the Hippocampus
Elena Blanco-Suárez
5. Bilateral Carotid Artery Stenosis and Cerebral Blood Flow Outcomes
Ifechukwude Joachim Biose and Gregory Jaye Bix
6. Internal Carotid Artery Stenosis: A Surgical Mouse Model to Study Moyamoya Syndrome
Jill M. Roberts and Justin F. Fraser
7. Modeling Distal Middle Cerebral Artery Occlusion in Neonatal Rodents with Magnetic Nanoparticles or Magnetized Red Blood Cells
Jie-Min Jia and Yuxiao Jin
Part II: In Vivo and Post-Mortem Imaging of Mechanisms of Recovery
8. In Vivo Imaging of the Structural Plasticity of Cortical Neurons after Stroke
Emilia Conti, Francesco Saverio Pavone, and Anna Letizia Allegra Mascaro
9. Measurement of Uninterrupted Cerebral Blood Flow by Laser Speckle Contrast Imaging (LSCI) during the Mouse Middle Cerebral Artery Occlusion Model by an Inverted LSCI Setup
Sung-Ha Hong, Andrea Doan, and Sean P. Marrelli
10. Multi-Exposure Speckle Imaging for Quantitative Evaluation of Cortical Blood Flow
Adam Santorelli, Colin T. Sullender, and Andrew K. Dunn
11. Wide-Field Optical Imaging in Mouse Models of Ischemic Stroke
Jonah A. Padawer-Curry, Ryan M. Bowen, Anmol Jarang, Xiaodan Wang, Jin-Moo Lee, and Adam Q. Bauer
12. Post-Mortem Magnetic Resonance Imaging of Degenerating and Reorganizing White Matter in Post-Stroke Rodent Brain
Vera H. Wielenga, Rick M. Dijkhuizen, and Annette Van der Toorn
Part III: Methods to Identify Molecular and Immune Mechanisms Supporting Recovery
13. Quantitative Spatial Mapping of Axons across Cortical Regions to Assess Axonal Sprouting after Stroke
Mary T. Joy, Samuel P. Bridges, and S. Thomas Carmichael
14. Quantitative Evaluation of Cerebral Microhemorrhages in the Mouse Brain
Rudy Chang and Rachita K. Sumbria
15. In Vivo Evaluation of BBB Integrity in the Post-Stroke Brain
Yong Zhang, Saeideh Nozohouri, and Thomas J. Abbruscato
16. High Resolution RNA Sequencing from PFA-Fixed Microscopy Sections
Hao Ji, Simon Besson-Girard, Peter Androvic, Buket Bulut, Lu Liu, Yijing Wang, and Ozgun Gokce
17. FACS to Identify Immune Subsets in Mouse Brain and Spleen
Mary K. Malone, Thomas A. Ujas, Katherine M. Cotter, Daimen Britsch, Jenny Lutshumba, Jadwiga-Turchan-Cholewo, and Ann M. Stowe
18. A Guide on Analyzing Flow Cytometry Data Using Clustering Methods and Non-Linear Dimensionality Reduction (tSNE or UMAP)
Thomas A. Ujas, Veronica Obregon-Perko, and Ann M. Stowe
19. Co-Culturing Immune Cells and Mouse-Derived Mixed Cortical Cultures with Oxygen-Glucose Deprivation to In Vitro Simulate Neuroinflammatory Interactions after Stroke
Thomas A. Ujas, Vanessa O. Torres, and Ann M. Stowe
Part IV: Behavioral Methods for Quantifying Functional Recovery
20. Assessing Depression and Cognitive Impairment Following Stroke and Neurotrauma: Behavioral Methods for Quantifying Impairment and Functional Recovery
Karienn A. de Souza, Michelle Hook, and Farida Sohrabji
21. Use of an Automated Mouse Touchscreen Platform for Quantification of Cognitive Deficits after Central Nervous System Injury
Katherine M. Cotter, Grace L. Bancroft, Harley A. Haas, Raymon Shi, Andrew N. Clarkson, Matthew E. Croxall, Ann M. Stowe, Sanghee Yun, and Amelia J. Eisch
22. Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use after Stroke
Dene M. Betz, April M. Becker, Katherine M. Cotter, Andrew M. Sloan, Ann M. Stowe, and Mark P. Goldberg
23. The Finer Aspects of Grid-Walking and Cylinder Tests for Experimental Stroke Recovery Studies in Mice
Nausheen Syeara, Sounak Bagchi, Abdullah Al Shoyaib, Serob T. Karamyan, Faisal F. Alamri, and Vardan T. Karamyan
24. Performing Enriched Environment Studies to Improve Functional Recovery
Daniela Talhada and Karsten Ruscher
Part V: Neurotherapeutics and Functional Recovery
25. Clinically-Applicable Experimental Design and Considerations for Stroke Recovery Preclinical Studies
Vardan T. Karamyan
26. Hydrogels and Nanoscaffolds for Long-Term Intraparenchymal Therapeutic Delivery after Stroke
Mozammel H. Bhuiyan, Josh Houlton, and Andrew N. Clarkson
27. Reverse Translation to Develop Post-Stroke Therapeutic Interventions during Mechanical Thrombectomy: Lessons from the BACTRAC Trial
Benton Maglinger, Jacqueline A. Frank, Justin F. Fraser, and Keith R. Pennypacker
28. Methods to Study Drug Uptake at the Blood-Brain Barrier Following Experimental Ischemic Stroke: In Vitro and In Vivo Approaches
Robert D. Betterton, Erica I. Williams, Kelsy L. Nilles, Thomas P. Davis, and Patr
