Neural Progenitor Cells Methods and Protocols

This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Culturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition

            Hassan Azari

 

2. Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region

            Alessandra Ricca, Federica Cascino, and Angela Gritti

 

3. Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres

            Ruslan Rust and Tara L. Walker

 

4. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord

            Jean-Philippe Hugnot

 

5. Culturing and Expansion of “Clinical Grade” Neural Stem Cells from the Fetal Human Central Nervous System

            Maurizio Gelati, Daniela Celeste Profico, Daniela Ferrari, and Angelo Luigi Vescovi

 

6. Microglia Isolation from Neural Stem Cell-Enriched Regions

            Gregory P. Marshall II

 

7. Microglial FACS Sorting for Robust RNA Recovery for Next Generation Sequencing

            Andria L. Doty and Aline Oliveria

 

8. Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded under Neurosphere Culture

            Nasser Nassiri Koopaei, Thomas D. Schmittgen, Brent A. Reynolds, and Hassan Azari

 

9. Isolating and Culturing of Precursor Cells from the Adult Human Brain

            Florian A. Siebzehnrubl

 

10. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord

            Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahaye, Alain Boularan, Alain Privat, and Jean-Philippe Hugnot

 

11. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny

            Hassan Azari

 

12. Identifying Neural Progenitor Cells in the Adult Human Brain

            Thomas I.H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow, and Maurice A. Curtis

 

13. Neonatal Transplant in Hypoxic Injury

            Tong Zheng and Michael D. Weiss

 

14. Isolation and Purification of Self Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke

            Marcel M. Daadi

 

15. Generating Cerebral Organoids from Human Pluripotent Stem Cells

            Leon Chew, Adam Añonuevo, and Erin Knock

 

16. Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue

            Maurice Curtis and Vinata Vedam-Mai

 

17. Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases

            Vinata Vedam-Mai