Neural Progenitor Cells Methods and Protocols

This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Culturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition.- Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region.- Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres.- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord.- Culturing and Expansion of “Clinical Grade” Neural Stem Cells from the Fetal Human Central Nervous System.- Microglia Isolation from Neural Stem Cell-Enriched Regions.- Microglial FACS Sorting for Robust RNA Recovery for Next Generation Sequencing.- Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded under Neurosphere Culture.- Isolating and Culturing of Precursor Cells from the Adult Human Brain.- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord.- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny.- Identifying Neural Progenitor Cells in the Adult Human Brain.- Neonatal Transplant in Hypoxic Injury.- Isolation and Purification of Self Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke.- Generating Cerebral Organoids from Human Pluripotent Stem Cells.- Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue.- Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.