Mouse Retinal Phenotyping Methods and Protocols

The aim of this volume is to provide a wide range of techniques and aspects of mouse retinal phenotyping and characterization. Chapters guide readers through morphological and functional properties of the mouse retina and techniques for the analysis of outer to mid-retina and mid- to inner retina. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Mouse Retinal Phenotyping: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Morphological Survey from Neurons to Circuits of the Mouse Retina.- Measuring Retinal Function in the Mouse.- Modeling Retinal Diseases Using Genetic Approaches in Mice.- Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells.- Two-photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (Flim) of Retinal Pigment Epithelium (RPE) of Mice In Vivo.- RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models.- Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models.- Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic .- Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System In Retinal Rods of the Mouse.- Microglia Analysis in Retinal Degeneration Mouse Models.- Determination Of Mitochondrial Oxygen Consumption in the Retina Ex-vivo: Applications for Retinal Disease.- Analysis of Feedback Signaling from Horizontal Cells to Photoreceptors in Mice.- Assessment of the Absolute Excitatory Level of the Retina by Flicker Erg.- Ex Vivo Functional Evaluation of Synaptic Transmission From Rods to Rod Bipolar Cells In Mice.- Functional And Morphological Analysis of Off Bipolar Cells.- Immunohistochemical Phenotyping Of Mouse Amacrine Cell Subtypes.- Phenotyping of Gap-Junctional Coupling in the Mouse Retina.- Ganglion Cell Assessment in Rodents with Retinal Degeneration.- Morphological Identification of Melanopsin Expressing Retinal Ganglion Cell Subtypes in Mice.- Functional Assessment of Melanopsin-driven Light Responses in the Mouse: Multielectrode Array Recordings.- In Vitro Assays for Mouse Müller Cell Phenotyping through Microrna Profiling in the Damaged Retina.- Analysis Of Retinal Vascular Plexi and Interplexus Connections.