Molecular Virology Methods and Protocols

This detailed book presents classical and cutting-edge techniques in molecular virology research. The volume begins with guidelines for investigating viral structure, proliferation processes, and virulence factors, followed by various approaches to identify key elements and molecular signaling pathways in virus-infected host cells that regulate virus-host interactions. Additional chapters explore methods for studying autophagy, post-translational modifications of virus and host proteins, epigenetic factors in anti-viral immune responses, and viral epidemiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Virology: Methods and Protocols serves as an ideal guide to this vitally important area of study.

Investigation of Protein Fatty Acylation in Virus-Host Interaction.- Determination of Protein Ubiquitination after Protein Denaturation in Virus-Host Interactions.- Viral Circuit Tracing in Biomedical Research: Methods, Protocols, and Applications.- Analysis of the Virus-Host Protein-Protein Interaction via Co-Immunoprecipitation (Co-IP).- Tandem Affinity Purification of Virus-Host Protein Complexes via the Strep/FLAG (SF)-TAP Tag.- Applying 3D Genome Technology in Virology Research.- In Vitro Liquid-Liquid Phase Separation Assay for Viral Proteins.- Computational Analysis of Virus-Host Interactomes.- Application of Single-Cell RNA Sequencing in Investigating Virus-Host Interactions.- CRISPR-Mediated Viral Gene Knock-In for Studying Viral-Host Interactions.- High-Throughput Yeast Two-Hybrid Technology for Investigating Molecular Virology.- Pull-Down Techniques for Determining Virus-Host Protein Interactions.- Application of Organoids to Investigate Virus-Host Interactions.- Identification of Virus-Host Protein Interactions via Proteomic Techniques.- Engineering of Vesicular Stomatitis Virus (VSV) for Modulating Cell Death and Antitumor Immunity.- 3D Tissue Culture Model for Virology Studies.- Detection of Protein Interactions During Viral Infection by Bimolecular Fluorescence Complementation.- Engineering of the WSN Strain for Investigating Antiviral and Antitumor Immunity.- The Application of HSV-1 in Investigating Neurological Diseases.- Strategy and Application of Viral Potency Assays in Cell and Gene Therapy.- Chromatin Immunoprecipitation (ChIP)-Quantitative PCR (qPCR) Analysis of Viral Genome-Host Protein Interactions.- The Application of Base Editing Technology to Investigate Virus-Host Interactions and Antiviral Therapeutic Strategies.- Zebrafish as a Model for Studying Virus-Host Interactions.- Screening for Host Proteins with Pro- and Antiviral Activity via High-Throughput CRISPR.- Isotope Labeling Techniques for Virus-Infected Cells.- Reverse Genetics-Based Methodology for Molecular Virology Study.- RBD Amplicon Sequencing and Evolutionary Analysis of SARS-CoV-2 Variants from Wastewater Samples.- RNA Binding Protein Immunoprecipitation 1 (RIP) Assay to Investigate the Interactions Between Viral RNA and Host Proteins.- Dual-Luciferase Reporter Assay to Investigate Virus-Host Interactions.- Electrophoretic Mobility Shift Assay for Identification of the Interaction Between a Viral Protein and DNA.- Applying Phage Display Technology to Obtain Specific Peptides Blocking Host-Virus Interactions.- Single Particle Cryo-EM to Investigate Nonenveloped Virus-Like Particles.- SELEX (Systematic Evolution of Ligands by Exponential Enrichment) to Identify DNA Motifs Mirroring Chromosome Targets of the Hypothetical Transcription Factor NLRP3.- Detection of Protein Interactions and Dynamics During Viral Infection via Fluorescence Resonance Energy Transfer.