Molecular Engineering Principles and Applications

This book is designed to help students interested in biomedical engineering learn the essential concepts of biology and molecular biology. Serving as a practical guide to molecular engineering, the book will help students realize the unique approach they can take to tackle modern biomedical problems and challenges, given their training in physics, mathematics, chemistry, and other quantitative topics.

Fundamentals. Biological Information Flow Replication, Transcription, Translation, Molecular Players. Structure and Function of Nucleic Acids and Proteins, Components and Physical/Chemical Properties, Structure Determination: X-ray Crystallography & NMR. Practice in Molecular Engineering and Validation. DNA Recombinant Technology Primer Design, Vector Design. Rational Design of Molecules, Structure and Function, Force Fields, Computational Tools. Directed Evolution Platforms Phage, Yeast, SELEX: Aptamers. Techniques for Molecular Interaction Studies, SPR, ITC, Gel Filtration, Mass-Spec, Gel Shift (EMSA). Applications. Antibody Engineering (structure, function etc.). Virus Engineering, Gene Delivery by Virus and Virus-Like Particles. Application to Diagnosis and Therapy, Whole Body Imaging Techniques: MRI, CT, PET, Optical Imaging, Conjugation Chemistry for Therapy and Diagnosis, Engineering Considerations for PK and PD, Immunotherapy.

Moonsoo M. Jin, Sc.D., is a faculty member in the Department of Radiology at Weill Cornell Medical College, New York, New York, USA.