Molecular Endocrinology Methods and Protocols

Over the course of the last hundred years, the field of endocrinology has received ever-increasing attention. Research and developing technologies have resulted in a significant leap in our understanding of the causes and treatment of endocrine dysfunction. In Molecular Endocrinology: Methods and Protocols, leaders in the field share a diversity of cutting-edge techniques that are becoming routinely employed in the quest to further understand hormone action.  In order to reach the widest number of labs, this volume includes protocols that allow investigators at all stages of their scientific career to successfully perform these techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

Comprehensive and easy to use, Molecular Endocrinology: Methods and Protocols is an ideal guide for researchers in labs across the world who seek a deeper understanding of the complex processes and biology of hormones.

Determining the Affinity of Hormone?Receptor Interaction.- Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques.- Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and Their Functional Consequences at the Hormone–Receptor Interaction Level.- Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane.- Gene Expression Profiling in the Aging Ovary.- Genomics Analysis: Endometrium.- Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization.- Adenoviral Gene Transfer into Isolated Pancreatic Islets.- Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes.- Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines.- Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells.- Use of Reporter Genes to Study Promoters of the Androgen Receptor.- Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor ?.- Chromosome-Wide Analysis of Protein Binding and Modifications.- Genome-Wide Analysis for Protein?DNA Interaction: ChIP-Chip.- Detection of ER?-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer.- Detection of Proteins Sumoylated In Vivo and In Vitro.- Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE).- Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis.- Reporter Mice for the Study of Intracellular Receptor Activity.- Real-Time Non-invasive Imaging of ES Cell-Derived Insulin Producing Cells.- Transgenic Mouse Technology: Principles and Methods.- Breast Tumor-InitiatingCells Isolated from Patient Core Biopsies for Study of Hormone Action.- Markers of Oxidative Stress and Sperm Chromatin Integrity.- Planning and Executing a Genome Wide Association Study (GWAS).